Comparative Genomic and Functional Analysis of 100 Lactobacillus rhamnosus Strains and Their Comparison with Strain GG

PLoS Genetics - Tập 9 Số 8 - Trang e1003683
François P. Douillard1, Angela Ribbera1,2, Ravi Kant1, Taija E. Pietilä1, Hanna Järvinen3, Marcel Messing3, Cinzia Lucia Randazzo4, Lars Paulín5, Pia Laine5, Jarmo Ritari1, Cinzia Caggia4, Tanja Lähteinen1, Stan J. J. Brouns2, Reetta Satokari1,6, Ingemar von Ossowski1, Justus Reunanen1, Airi Palva1, Willem M. de Vos1,3,2
1Department of Veterinary Biosciences, University of Helsinki, Helsinki, Finland
2Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
3Infection Biology Program, Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
4Department of Agri-Food and Environmental System Management, University of Catania, Catania, Italy
5Institute of Biotechnology, University of Helsinki, Helsinki, Finland
6Functional Foods Forum, University of Turku, Turku, Finland

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C Clemente Jose, 2012, The impact of the gut microbiota on human health: an integrative view, Cell, 148, 1258, 10.1016/j.cell.2012.01.035

M Rajilić-Stojanović, 2007, Diversity of the human gastrointestinal tract microbiota revisited, Environ Microbiol, 9, 2125, 10.1111/j.1462-2920.2007.01369.x

SJ Williamson, 2011, From bacterial to microbial ecosystems (metagenomics) Bacterial Molecular Networks, Meth Mol Biol, 35

EE Vaughan, 2000, A molecular view of the intestinal ecosystem, Curr Issues Intest Microbiol, 1, 1

M Kleerebezem, 2009, Probiotic and gut lactobacilli and bifidobacteria: molecular approaches to study diversity and activity, Annu Rev Microbiol, 63, 269, 10.1146/annurev.micro.091208.073341

EG Zoetendal, 2012, The human small intestinal microbiota is driven by rapid uptake and conversion of simple carbohydrates, ISME J, 6, 1415, 10.1038/ismej.2011.212

MJ Claesson, 2009, Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine, PLoS ONE, 4, e6669, 10.1371/journal.pone.0006669

A Salonen, 2012, The adult intestinal core microbiota is determined by analysis depth and health status, Clin Microbiol Infect, 4, 16, 10.1111/j.1469-0691.2012.03855.x

HG Heilig, 2002, Molecular diversity of <italic>Lactobacillus</italic> spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA, Appl Environ Microbiol, 68, 114, 10.1128/AEM.68.1.114-123.2002

J Walter, 2008, Ecological Role of Lactobacilli in the gastrointestinal tract: implications for fundamental and biomedical research, Appl Environ Microbiol, 74, 4985, 10.1128/AEM.00753-08

J Walter, 2010, Host-microbial symbiosis in the vertebrate gastrointestinal tract and the <italic>Lactobacillus reuteri</italic> paradigm, Proc Natl Acad Sci U S A, 108 Suppl 1, 4645

M Kalliomaki, 2001, Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial, Lancet, 357, 1076, 10.1016/S0140-6736(00)04259-8

M Kalliomaki, 2003, Probiotics and prevention of atopic disease: 4-year follow-up of a randomised placebo-controlled trial, Lancet, 361, 1869, 10.1016/S0140-6736(03)13490-3

M Bernardeau, 2006, Beneficial lactobacilli in food and feed: long-term use, biodiversity and proposals for specific and realistic safety assessments, FEMS Microbiol Rev, 30, 487, 10.1111/j.1574-6976.2006.00020.x

S Lebeer, 2010, Adaptation factors of the probiotic <italic>Lactobacillus rhamnosus</italic> GG, Benef Microbes, 1, 335, 10.3920/BM2010.0032

M Saxelin, 2005, Probiotic and other functional microbes: from markets to mechanisms, Curr Opin Biotechnol, 16, 204, 10.1016/j.copbio.2005.02.003

CL Randazzo, 2007, Preliminary characterization of wild lactic acid bacteria and their abilities to produce flavour compounds in ripened model cheese system, J Appl Microbiol, 103, 427, 10.1111/j.1365-2672.2006.03261.x

I Pitino, 2012, Survival of <italic>Lactobacillus rhamnosus</italic> strains inoculated in cheese matrix during simulated human digestion, Food Microbiol, 31, 57, 10.1016/j.fm.2012.02.013

M Kankainen, 2009, Comparative genomic analysis of <italic>Lactobacillus rhamnosus</italic> GG reveals pili containing a human-mucus binding protein, Proc Natl Acad Sci U S A, 106, 17193, 10.1073/pnas.0908876106

J Reunanen, 2012, Characterization of the SpaCBA pilus fibers in the probiotic <italic>Lactobacillus rhamnosus</italic> GG, Appl Environ Microbiol, 78, 2337, 10.1128/AEM.07047-11

I von Ossowski, 2010, Mucosal adhesion properties of the probiotic <italic>Lactobacillus rhamnosus</italic> GG SpaCBA and SpaFED pilin subunits, Appl Environ Microbiol, 76, 2049, 10.1128/AEM.01958-09

MR Millar, 1993, Enteral feeding of premature infants with <italic>Lactobacillus</italic> GG, Arch Dis Child, 69, 483, 10.1136/adc.69.5_Spec_No.483

BW Petschow, 2005, Effects of feeding an infant formula containing <italic>Lactobacillus</italic> GG on the colonization of the intestine: a dose-response study in healthy infants, J Clin Gastroenterol, 39, 786, 10.1097/01.mcg.0000177245.53753.86

M Alander, 1999, Persistence of colonization of human colonic mucosa by a probiotic strain, <italic>Lactobacillus rhamnosus</italic> GG, after oral consumption, Appl Environ Microbiol, 65, 351, 10.1128/AEM.65.1.351-354.1999

S Lebeer, 2008, Genes and molecules of lactobacilli supporting probiotic action, Microbiol Mol Biol Rev, 72, 728, 10.1128/MMBR.00017-08

DR Mack, 2003, Extracellular MUC3 mucin secretion follows adherence of <italic>Lactobacillus</italic> strains to intestinal epithelial cells in vitro, Gut, 52, 827, 10.1136/gut.52.6.827

S Vesterlund, 2006, <italic>Staphylococcus aureus</italic> adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria, Microbiology, 152, 1819, 10.1099/mic.0.28522-0

KC Johnson-Henry, 2008, <italic>Lactobacillus rhamnosus</italic> strain GG prevents enterohemorrhagic <italic>Escherichia coli</italic> O157:H7-induced changes in epithelial barrier function, Infect Immun, 76, 1340, 10.1128/IAI.00778-07

VB Young, 2012, The intestinal microbiota in health and disease, Curr Opin Gastroenterol, 28, 63, 10.1097/MOG.0b013e32834d61e9

S Lebeer, 2010, Host interactions of probiotic bacterial surface molecules: comparison with commensals and pathogens, Nat Rev Microbiol, 8, 171, 10.1038/nrmicro2297

TR Klaenhammer, 2012, The impact of probiotics and prebiotics on the immune system, Nat Rev Immunol, 12, 728, 10.1038/nri3312

M Callanan, 2008, Genome sequence of <italic>Lactobacillus helveticus</italic>, an organism distinguished by selective gene loss and insertion sequence element expansion, J Bacteriol, 190, 727, 10.1128/JB.01295-07

H Cai, 2009, Genome sequence and comparative genome analysis of <italic>Lactobacillus casei</italic>: insights into their niche-associated evolution, Genome Biol Evol, 1, 239, 10.1093/gbe/evp019

R Siezen, 2010, Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches, Environ Microbiol, 12, 758, 10.1111/j.1462-2920.2009.02119.x

R Siezen, 2011, Genomic diversity and versatility of <italic>Lactobacillus plantarum</italic>, a natural metabolic engineer, Microbial Cell Fact, 10, S3, 10.1186/1475-2859-10-S1-S3

M Succi, 2005, Bile salt and acid tolerance of <italic>Lactobacillus rhamnosus</italic> strains isolated from Parmigiano Reggiano cheese, FEMS Microbiol Lett, 244, 129, 10.1016/j.femsle.2005.01.037

MK Salminen, 2002, Lactobacillus bacteremia during a rapid Increase in probiotic use of <italic>Lactobacillus rhamnosus</italic> GG in Finland, Clin Infect Dis, 35, 1155, 10.1086/342912

LM Pascual, 2008, <italic>Lactobacillus rhamnosus</italic> L60, a potential probiotic isolated from the human vagina, J Gen Appl Microbiol, 54, 141, 10.2323/jgam.54.141

B Richard, 2001, Identification of salivary <italic>Lactobacillus rhamnosus</italic> species by DNA profiling and a specific probe, Res Microbiol, 152, 157, 10.1016/S0923-2508(01)01187-1

O O'Sullivan, 2009, Comparative genomics of lactic acid bacteria reveals a niche-specific gene set, BMC Microbiol, 9, 50, 10.1186/1471-2180-9-50

J Broadbent, 2012, Analysis of the <italic>Lactobacillus casei</italic> supragenome and its influence in species evolution and lifestyle adaptation, BMC Genomics, 13, 533, 10.1186/1471-2164-13-533

GL Douglas, 2010, Genomic evolution of domesticated microorganisms, Annu Rev Food Sci Technol, 1, 397, 10.1146/annurev.food.102308.124134

K Koskenniemi, 2011, Proteomics and transcriptomics characterization of bile stress response in probiotic <italic>Lactobacillus rhamnosus</italic> GG, Mol Cell Prot, 10, M110.002741, 10.1074/mcp.M110.002741

SCJ De Keersmaecker, 2006, Strong antimicrobial activity of <italic>Lactobacillus rhamnosus</italic> GG against <italic>Salmonella typhimurium</italic> is due to accumulation of lactic acid, FEMS Microbiol Lett, 259, 89, 10.1111/j.1574-6968.2006.00250.x

SA Frese, 2011, The Evolution of host specialization in the vertebrate gut symbiont <italic>Lactobacillus reuteri</italic>, PLoS Genet, 7, e1001314, 10.1371/journal.pgen.1001314

H Morita, 2009, Complete genome sequence of the probiotic <italic>Lactobacillus rhamnosus</italic> ATCC 53103, J Bacteriol, 191, 7630, 10.1128/JB.01287-09

V Pittet, 2012, Genome Sequence of Lactobacillus rhamnosus ATCC 8530, J Bacteriol, 194, 726, 10.1128/JB.06430-11

W de Vos, 2011, Systems solutions by lactic acid bacteria: from paradigms to practice, Microb Cell Fact, 10, S2, 10.1186/1475-2859-10-S1-S2

H Tettelin, 2005, Genome analysis of multiple pathogenic isolates of <italic>Streptococcus agalactiae</italic>: Implications for the microbial “pan-genome”, Proc Natl Acad Sci U S A, 102, 13950, 10.1073/pnas.0506758102

R Kant, 2011, Comparative genomics of <italic>Lactobacillus</italic>, Microb Biotechnol, 4, 323, 10.1111/j.1751-7915.2010.00215.x

R Jansen, 2002, Identification of genes that are associated with DNA repeats in prokaryotes, Mol Microbiol, 43, 1565, 10.1046/j.1365-2958.2002.02839.x

P Horvath, 2008, Diversity, activity, and evolution of CRISPR loci in <italic>Streptococcus thermophilus</italic>, J Bacteriol, 190, 1401, 10.1128/JB.01415-07

H Deveau, 2008, Phage response to CRISPR-encoded resistance in <italic>Streptococcus thermophilus</italic>, J Bacteriol, 190, 1390, 10.1128/JB.01412-07

R Barrangou, 2007, CRISPR provides acquired resistance against viruses in prokaryotes, Science, 315, 1709, 10.1126/science.1138140

LA Marraffini, 2008, CRISPR Interference limits horizontal gene transfer in <italic>Staphylococci</italic> by targeting DNA, Science, 322, 1843, 10.1126/science.1165771

LA Marraffini, 2010, CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea, Nat Rev Genet, 11, 181, 10.1038/nrg2749

ER Westra, 2012, The CRISPRs, they are A-changin&apos;: how prokaryotes generate adaptive immunity, Annu Rev Genet, 46, 311, 10.1146/annurev-genet-110711-155447

J Zhang, 2010, <italic>Mycobacterium tuberculosis</italic> complex CRISPR genotyping: improving efficiency, throughput and discriminative power of ‘spoligotyping’ with new spacers and a microbead-based hybridization assay, J Med Microbiol, 59, 285, 10.1099/jmm.0.016949-0

S Delannoy, 2012, Use of CRISPR sequence polymorphisms for specific detection of enterohemorrhagic <italic>E. coli</italic> (EHEC) strains of serotypes O26:H11, O45:H2, O103:H2, O111:H8, O121:H19, O145:H28 and O157:H7 by real time PCR, J Clin Microbiol, 12, 4035, 10.1128/JCM.02097-12

L Fabre, 2012, CRISPR typing and subtyping for improved laboratory surveillance of <italic>Salmonella</italic> infections, PLoS ONE, 7, e36995, 10.1371/journal.pone.0036995

P Horvath, 2009, Comparative analysis of CRISPR loci in lactic acid bacteria genomes, Int J Food Microbiol, 131, 62, 10.1016/j.ijfoodmicro.2008.05.030

N El Aila, 2009, Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora, BMC Infect Dis, 9, 167, 10.1186/1471-2334-9-167

FP Douillard, 2013, Comparative genomic and functional analysis of <italic>Lactobacillus casei</italic> and <italic>Lactobacillus rhamnosus</italic> strains marketed as probiotics, Appl Environ Microbiol, 79, 1923, 10.1128/AEM.03467-12

W Sybesma, 2013, Genome instability in <italic>Lactobacillus rhamnosus</italic> GG, Appl Environ Microbiol, 79, 2233, 10.1128/AEM.03566-12

S Lebeer, 2012, Functional analysis of <italic>Lactobacillus rhamnosus</italic> GG pili in relation to adhesion and immunomodulatory interactions with intestinal epithelial cells, Appl Environ Microbiol, 78, 185, 10.1128/AEM.06192-11

RJ Hickey, 2012, Understanding vaginal microbiome complexity from an ecological perspective, Transl Res, 160, 267, 10.1016/j.trsl.2012.02.008

E Poznanski, 2004, Indigenous raw milk microbiota influences the bacterial development in traditional cheese from an alpine natural park, Int J Food Microbiol, 92, 141, 10.1016/j.ijfoodmicro.2003.09.006

M Silva, 1987, Antimicrobial substance from a human <italic>Lactobacillus</italic> strain, Antimicrob Agents Chemother, 31, 1231, 10.1128/AAC.31.8.1231

EM Lehto, 1997, Inhibition of <italic>Salmonella typhimurium</italic> adhesion to Caco-2 cell cultures by <italic>Lactobacillus</italic> strain GG spent culture supernate: only a pH effect?, FEMS Immunol Med Microbiol, 18, 125, 10.1111/j.1574-695X.1997.tb01037.x

H Brüssow, 2001, Phages of dairy bacteria, Annu Rev Microbiol, 55, 283, 10.1146/annurev.micro.55.1.283

EA Pfeiler, 2007, The genomics of lactic acid bacteria, Trends Microbiol, 15, 546, 10.1016/j.tim.2007.09.010

S-J Sheu, 2010, Use of <italic>tuf</italic> gene-based primers for the PCR detection of probiotic <italic>Bifidobacterium</italic> species and enumeration of <italic>Bifidobacteria</italic> in fermented milk by cultural and quantitative Real-Time PCR methods, J Food Sci, 75, M521, 10.1111/j.1750-3841.2010.01816.x

M Ventura, 2003, Analysis, characterization, and loci of the <italic>tuf</italic> genes in <italic>Lactobacillus</italic> and <italic>Bifidobacterium</italic> species and their direct application for species identification, Appli Environ Microbiol, 69, 6908, 10.1128/AEM.69.11.6908-6922.2003

H Li, 2009, The Sequence Alignment/Map format and SAMtools, Bioinformatics, 25, 2078, 10.1093/bioinformatics/btp352

S Kurtz, 2004, Versatile and open software for comparing large genomes, Genome Biol, 5, R12, 10.1186/gb-2004-5-2-r12

S Vesterlund, 2005, Measurement of bacterial adhesion— <italic>in vitro</italic> evaluation of different methods, J Microbiol Meth, 60, 225, 10.1016/j.mimet.2004.09.013

E Skyttä, 1991, A quantitative method for assessing bacteriocins and other food antimicrobials by automated turbidometry, J Microbiol Meth, 14, 77, 10.1016/0167-7012(91)90036-P

A Sturn, 2002, Genesis: cluster analysis of microarray data, Bioinformatics, 18, 207, 10.1093/bioinformatics/18.1.207

K Tamura, 2007, MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0, Mol Biol Evol, 24, 1596, 10.1093/molbev/msm092

MK Salminen, 2006, <italic>Lactobacillus</italic> bacteremia, species identification, and antimicrobial susceptibility of 85 blood isolates, Clinical Infect Dis, 42, e35, 10.1086/500214