Comparative Genome Sequence Analysis of Multidrug-Resistant Acinetobacter baumannii

Journal of Bacteriology - Tập 190 Số 24 - Trang 8053-8064 - 2008
Mark D. Adams1, Karrie Goglin1, Neil Molyneaux1, Kristine M. Hujer2, Heather Lavender1, Jennifer J. Jamison3, Ian J. MacDonald3, Kristienna M. Martin3, Thomas A. Russo4,5,3,6, Anthony A. Campagnari5,3, Andrea M. Hujer2, Robert A. Bonomo7,2, Steven R. Gill8,3
1Department of Genetics
2Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio
3New York State Center of Excellence in Bioinformatics and Life Sciences
4Department of Medicine
5Department of Microbiology & Immunology
6Veterans Administration Western New York Healthcare System
7Departments of Pharmacology and Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio
8Department of Oral Biology, The State University of New York at Buffalo, Buffalo, New York

Tóm tắt

ABSTRACT The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii isolates. The entire MDR phenotype can be explained by the acquisition of discrete resistance determinants distributed throughout the genome. A comparison of closely related MDR and drug-susceptible isolates suggests that drug efflux may be a less significant contributor to resistance to certain classes of antibiotics than inactivation enzymes are. A resistance island with a variable composition of resistance determinants interspersed with transposons, integrons, and other mobile genetic elements is a significant but not universal contributor to the MDR phenotype. Four hundred seventy-five genes are shared among all six clinical isolates but absent from the related environmental species Acinetobacter baylyi ADP1. These genes are enriched for transcription factors and transporters and suggest physiological features of A. baumannii that are related to adaptation for growth in association with humans.

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Journal of Bacteriology

Tập 190 Số 24

8053-8064

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