Combining selected immunomodulatory <i>Propionibacterium freudenreichii</i> and <i>Lactobacillus delbrueckii</i> strains: Reverse engineering development of an anti‐inflammatory cheese

Molecular Nutrition and Food Research - Tập 60 Số 4 - Trang 935-948 - 2016
Coline Plé1, Jérôme Breton1, Romain Richoux2, Marine Nurdin2, Stéphanie-Marie Deutsch3, Hélène Falentin3, Christine Hervé4, Victoria Chuat3, Riwanon Lemée4, Emmanuelle Maguin5, Gwénaël Jan3, Benoît Foligné1
1Lactic Acid Bacteria & Mucosal Immunity - CIIL
2Actalia - division produits laitiers
3Science et Technologie du Lait et de l'Oeuf
4Laboratoires Standa
5Département Microbiologie et Chaîne Alimentaire

Tóm tắt

Scope: Inflammatory bowel disease (IBD) constitutes a growing public health concern in western countries. Bacteria with anti‐inflammatory properties are lacking in the dysbiosis accompanying IBD. Selected strains of probiotic bacteria with anti‐inflammatory properties accordingly alleviate symptoms and enhance treatment of ulcerative colitis in clinical trials. Such properties are also found in selected strains of dairy starters such as Propionibacterium freudenreichii and Lactobacillus delbrueckii (Ld). We thus investigated the possibility to develop a fermented dairy product, combining both starter and probiotic abilities of both lactic acid and propionic acid bacteria, designed to extend remissions in IBD patients.Methods and results: We developed a single‐strain Ld‐fermented milk and a two‐strain P. freudenreichii and Ld‐fermented experimental pressed cheese using strains previously selected for their anti‐inflammatory properties. Consumption of these experimental fermented dairy products protected mice against trinitrobenzenesulfonic acid induced colitis, alleviating severity of symptoms, modulating local and systemic inflammation, as well as colonic oxidative stress and epithelial cell damages. As a control, the corresponding sterile dairy matrix failed to afford such protection.Conclusion: This work reveals the probiotic potential of this bacterial mixture, in the context of fermented dairy products. It opens new perspectives for the reverse engineering development of anti‐inflammatory fermented foods designed for target populations with IBD, and has provided evidences leading to an ongoing pilot clinical study in ulcerative colitis patients.

Từ khóa


Tài liệu tham khảo

Ghouri Y. A., 2014, Systematic review of randomized controlled trials of probiotics, prebiotics, and synbiotics in inflammatory bowel disease, Clin. Exp. Gastroenterol., 7, 473

10.1099/00222615-36-2-96

Duboc H., Connecting dysbiosis, bile‐acid dysmetabolism and gut inflammation in inflammatory bowel diseases, Gut 2013, 62, 531

David L. A., Diet rapidly and reproducibly alters the human gut microbiome, Nature 2014, 505, 559

10.1016/j.ijfoodmicro.2015.03.015

10.3748/wjg.v13.i2.236

10.1111/j.1365-2036.2004.02268.x

10.1186/1472-6882-10-13

10.1016/S0140-6736(98)06343-0

10.1016/j.nut.2008.11.017

10.1155/2015/505878

10.1016/j.jff.2014.11.021

10.1371/journal.pone.0108360

10.3168/jds.2014-8041

10.1128/AEM.01976-10

10.1136/gut.2005.070490

Okada Y., 2006, 1.4‐dihydroxy‐2‐naphthoic acid (DHNA) shows anti‐inflammatory effect on NSAID‐induced colitis in IL‐10‐knockout mice through suppression of inflammatory cell infiltration and increased number of genus Bifidobacterium, Gastroenterology, 130, A313

10.1016/j.jprot.2014.07.018

10.1016/j.nut.2005.04.013

10.1371/journal.pone.0085923

10.1002/ibd.21834

10.1136/gutjnl-2012-304393

10.1111/j.1365-2672.1960.tb00188.x

10.1139/m68-199

10.1016/j.fm.2012.05.003

Madec M. N. Rouault A. Maubois J. L. Thierry A. INRA Milieu sélectif et procédé pour le dénombrement des bactéries propioniques. Brevet. FR. 2. 700. 778. ‐ A1. 1993 15.

10.1016/j.fm.2011.09.009

10.1016/j.clnu.2006.03.006

10.1016/0016-5085(89)90760-9

10.1136/gut.41.4.487

10.1111/1523-1747.ep12506462

10.1038/nmeth.f.303

10.1136/gut.2006.093484

10.1016/j.jnutbio.2013.01.006

10.1016/j.clnu.2014.11.008

10.1155/2014/932530

10.1021/jf302245m

10.1016/j.jff.2015.08.015

Falentin H. Deutsch S. M. Loux V. Hammani A.et al. Permanent draft genome of the probiotic strainPropionibacterium freudenreichiiCIRM‐BIA 129 (ITG P20).Stand. Genomic. Sci.2014 Accepted.

10.1128/genomeA.00328‐14

10.3168/jds.2013-7477

Wen L., Innate immunity and intestinal microbiota in the development of Type 1 diabetes, Nature 2008, 455, 1109

10.1128/AEM.01360‐15

10.1155/2015/723056

Thông tin
Thông tin xuất bản

Molecular Nutrition and Food Research

Tập 60 Số 4

935-948

Thông tin tác giả