Colonization of Rabbit Carcasses by Drain Fly Larvae, Psychoda sp. (Diptera: Psychodidae): The First Report
Tóm tắt
Psychoda sp. is often collected from patchy habitats such as sewers, drains and decomposing organic matters. The discovery of Psychoda sp. in forensic studies indicated that it might have noteworthy value in assisting death investigations. This study reports on the first finding of Psychoda larvae collected from decomposing rabbit carcasses placed in Cameron Highlands, Pahang, Malaysia. The larvae were first observed on rabbit carcasses and were collected using tweezers and carefully preserved in 70% ethanol. They were subsequently mounted on microscopy slides using Hoyer’s medium and identified as Psychoda sp. morphologically. The identification was also confirmed through a DNA barcoding analysis. Psychoda sp. larvae were collected on day-10 post-mortem where the rabbit carcasses were at the advanced decay stage of decomposition. The cytochrome c oxidase I (COI) gene sequences of the larvae had 90% similarity with the Psychoda spp. in the database. The finding of these larvae on carrion may provide additional valuable insights into forensic entomology and may assist in death investigations.
Tài liệu tham khảo
Salmela J, Kvifte GM, More A (2012) Description of a new Psychoda Latreille species from Fennoscandia (Diptera: Psychodidae). Zootaxa 3313:34–43
Griffith TB, Gillet-Kaufman J (2018) Drain fly Psychoda spp. (Insecta: Diptera:Psychodidae) (EENY-716). http://edis.ifas.ufl.edu/DLN. Accessed 18 Dec 2019
Quate LW (1962) The Psychodidae of Batu Caves, Malaya (Diptera). Pac Insects 4:219–234
Bravo F, Lopes PS, Bastos S (2010) A new Brazilian species of Psychoda Latreille, 1796 (Diptera, Psychodidae) collected in a bromeliad. Biota Neotrop 10:167–169. https://doi.org/10.1590/S1676-06032010000200021
Ahmad A, Ahmad AH, Dieng H, Satho T, Ahmad H, Aziz AT, Boots M (2011) Cadaver wrapping and arrival performance of adult flies in an oil palm plantation in northern peninsular Malaysia. J Med Entomol 48:1236–1246. https://doi.org/10.1603/ME10247
Heo CC, Nazni WA, Chew KW, Kurahashi H, Jeffery J, Heah SK, Omar B (2010) A study of cow dung diptera in Sentul Timur, Kuala Lumpur, Malaysia. J Trop Med Parasitol 33:53–61
Tüzün A, Dabiri F, Yüksel S (2010) Preliminary study and identification of insects’ species of forensic importance in Urmia, Iran. Afr J Biotechnol 9:3649–3658
Lindgren NK, Sisson MS, Archambeault AD, Rahlwes BC, Willett JR, Bucheli SR (2015) Four forensic entomology case studies: records and behavioral observations on seldom reported cadaver fauna with notes on relevant previous occurrences and ecology. J Med Entomol 52:143–150. https://doi.org/10.1093/jme/tju023
Redborg KE, Hinesly TD, Ziegler EL (1983) Rearing Psychoda alternata (Diptera: Psychodidae) in the laboratory on digested sewage sludge, with some observations on its biology. Environ Entomol 12:412–415. https://doi.org/10.1093/ee/12.2.412
Cordeiro DP, Wagner R (2018) Family Psychodidae. In: Thorp JH, Covich AP (eds) Thorp and Covich’s freshwater invertebrates. Academic Press, London, pp 765–770
Wagner R, Richardson BA, Richardson MJ (2008) A new psychodid species from Puerto Rican tank bromeliads. Stud Neotrop Fauna Envion 43:209–216. https://doi.org/10.1080/01650520802288173
Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PDN (2006) DNA barcodes distinguish species of tropical Lepidoptera. Proc Natl Acad Sci 103:968–971. https://doi.org/10.1073/pnas.0510466103
Turner CL (1923) The Psychodidae (moth-like flies) as subjects for studies in breeding and heredity. Am Nat 57:545–558. https://www.journals.uchicago.edu/doi/pdfplus/10.1086/279944
Bishop AL, McKenzie HJ, Barchia IM, Harris AM (1998) Occurrence and effect of temperature regimes on four species of fly (Diptera) found with ‘Culicoides brevitarsis’ Kieffer (Ceratopogonidae) in bovine dung. Gen Appl Entomol 28:93–99
Scott HG (1961) Filter fly control at sewage plants. Sanitarian 24:14–17
Munstermann LE (2019) Phlebotomine sand flies and moth flies (Psychodidae). In: Mullen GR, Durden L (eds) Medical and veterinary entomology. Academic Press, London, pp 191–211