Coccidioides posadasii infection alters the expression of pulmonary surfactant proteins (SP)-A and SP-D
Tóm tắt
Coccidioidomycosis or Valley Fever is caused by Coccidioides in Southwest US and Central America. Primary pulmonary infection is initiated by inhalation of air-borne arthroconidia. Since, lung is the first organ that encounters arthroconidia, different components of the pulmonary innate immune system may be involved in the regulation of host defense. Pulmonary surfactant proteins (SP)-A and SP-D have been recognized to play an important role in binding and phagocytosis of various microorganisms, but their roles in Coccidioides infection are not known. In this study, we studied the changes in amounts of pulmonary SP-A, SP-D and phospholipid in murine model of Coccidioides posadasii infection, and binding of SP-A and SP-D to Coccidioidal antigens. Mice were challenged intranasally with a lethal dose of C. posadasii (n = 30 arthroconidia) and bronchoalveolar lavage fluid (BALF) samples were collected on day 10, post infection. In another group of animals, mice were immunized with protective formalin killed spherule (FKS) vaccine prior to infection. The concentrations of BALF SP-A, SP-D, total phospholipid were measured using enzyme linked immunosorbent assay and biochemical assays. We found that in lavage fluid samples of C. posadasii infected mice, the concentrations of total phospholipid, SP-A and SP-D were 17 % (SEM 3.5, p < 0.001), 38 % (SEM 5.8, p < 0.001) and 4 % (SEM 1.3, p < 0.001) of those in lavage fluid samples of non-infected control mice, respectively. However, the concentrations of SP-A and SP-D remained unchanged in BALF samples of C. posadasii protected mice after immunization with FKS vaccine. Also, we found that both SP-A and SP-D bind to Coccidiodal antigens. Our results suggest that the C. posadasii infection perturbs the pulmonary SP-A, SP-D, and phospholipids, potentially enabling the disease progression and promoting fungal dissemination.
Tài liệu tham khảo
Fisher MC, Koenig GL, White TJ, Taylor JW: Molecular and phenotypic description of Coccidioides posadasii sp. Nov., previously recognized as the non-California population of Coccidioides immitis . Mycologia 2002, 94:73–84.
Pappagianis D: Epidemiology of coccidioidomycosis. In In Current topics in Medical Mycology. Volume 2. Edited by: McGinnis MR. New York: Springer Verlag; 1988:199–238.
Dixon DM: Coccidioides immitis as a select agent of bioterrorism. J Appl Microbiol 2001, 91:602–605.
Mischel PS, Vinters HV: Coccidioidomycosis of the central nervous system: neuropathological and vasculopathic manifestations and clinical correlates. Clin Infect Dis 1995, 20:400–405.
Beaman L, Pappagianis D, Benjamini E: Mechanism of resistance to infection with Coccidioides immitis in mice. Infect Immun 1979, 23:681–685.
Ampel NM, Christian L: In vitro modulation of proliferation and cytokine production by human peripheral blood mononuclear cells from subjects with various forms of coccidioidomycosis. Infect Immun 1997, 65:4483–4487.
King RJ: Pulmonary surfactant. J Appl Physiol 1982, 53:1–8.
Hoppe HJ, Reid KBM: Collectins, soluble proteins containing collagenous regions and lectin domains, and their roles in innate immunity. Protein Sci 1994, 3:1143–1158.
Holmskov U, Thiel S, Jensenius JC: Collectins and ficolins: Humoral lectins of the innate immune defense. Ann Rev Immunol 2003, 21:547–578.
Crouch EC: Surfactant protein-D and pulmonary host defense. Respir Res 2000, 1:93–108.
LeVine AM, Bruno MD, Huelsman KM, Ross GF, Whitsett JA, Korfhagen TR: Surfactant protein -A deficient mice are susceptible to group B streptococcal infection. J Immunol 1997, 158:4336–4340.
LeVine AM, Kurak KE, Bruno MD, Stark JM, Whitsett JA, Korfhagen TR: Surfactant protein -A deficient mice are susceptible to Pseudomonas aeruginosa infection. Am J Respir Cell Mol Biol 1998, 19:700–708.
Levine AM, Gwozdz J, Stark J, Bruno M, Whitsett JA, Korfhagen TR: Surfactant protein -A enhances repiratory syncytial virus clearance in vivo. J Clin Invest 1999, 103:1015–1021.
Hickling TP, Bright H, Wing K, Gower D, Martin SL, Sim RB, Malhotra R: A recombinant trimeric surfactant protein D carbohydrate recognition domain inhibits respiratory syncytial virus infection in vitro and in vivo. Eur J Immunol 1999, 29:3478–3484.
Wright JR: Immunomodulatory functions of surfactant. Physiol Rev 1997, 77:931–962.
Converse JL: Growth of spherules of Coccidioides immitis in a chemically defined liquid medium. Proc Soc Exp Biol Med 1955, 90:709–711.
Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959,37(8):911–7.
Stewart JC: Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Anal Biochem 1980, 104:10–14.
Pappagianis D, Smith CE, Kobayashi GS, Saito MT: Studies of antigens from young mycelia of Coccidioides immitis . J Infect Dis 1961, 108:35–44.
Awasthi S, Coalson JJ, Crouch E, Yang F, King RJ: Surfactant Proteins A and D in Premature Baboons with Chronic Lung Injury (Bronchopulmonary Dysplasia). Evidence for an Inhibition of Secretion. Am J Respir Crit Care Med 1999, 160:942–949.
Murakami S, Iwaki D, Mitsuzawa H, Sano H, Takahashi H, Voelker DR, Akino T, Kuroki Y: Surfactant protein A inhibts peptidoglycan-induced tumor necrosis factor-a secretion in U937 cells and alveolar macrophages by direct interaction with Toll-like receptor 2. J Biol Chem 2002, 277:6830–6837.
Jiang C, Magee DM, Cox RA: Coadministration of interleukin 12 expression vector with Antigen 2 cDNA enhances induction of protective immunity against Coccidioides immitis . Infect Immun 1999, 67:5848–5853.
O'Riordan DM, Standing JE, Kwon KY, Chang D, Crouch EC, Limper AH: Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages. J Clin Invest 1995, 95:2699–2710.
Schelenz S, Malhotra R, Sim RB, Holmskov U, Bancroft GJ: Binding of host collectins to the pathogenic yeast Cryptococcus neoformans: human surfactant protein D acts as an agglutinin for acapsular yeast cells. Infect Immun 1995, 63:3360–3366.
Madan T, Kishore U, Singh M, Strong P, Hussain EM, Reid KB, Sarma PU: Protective role of lung surfactant protein D in a murine model of invasive pulmonary aspergillosis. Infect Immun 2001, 69:2728–2731.
van Rozendaal BA, van Spriel AB, van De Winkel JG, Haagsman HP: Role of pulmonary surfactant D in innate defense against Candida albicans . J Infect Dis 2000, 182:917–922.
Madan T, Eggleton P, Kishore U, Strong P, Aggrawal SS, Sarma PU, Reid KB: Binding of pulmonary surfactant proteins A and D to Aspergillus fumigatus conidia enhances phagocytosis and killing by human neutrophils and alveolar macrophages. Infect Immun 1997, 65:3171–3179.
Gross NT, Camner P, Chinchilla M, Jarstrand C: In vitro effect of lung surfactant on alveolar macrophage defence mechanisms against Cryptococcus neoformans. Mycopathologia 1998, 144:21–27.
Sheehan PM, Stokes DC, Hughes WT: Surfactant phospholipids and lavage phospholipase A2 in experimental Pneumocystis carinii pneumonia. Am Rev Respir Dis 1986, 134:526–531.
Hoffman AG, Lawrence MG, Ognibene FP, Suffredini AF, Lipschik GY, Kovacs JA, Masur H, Shelhamer JH: Reduction of pulmonary surfactant in patients with human immunodeficiency virus infection and Pneumocystis carinii pneumonia. Chest 1992, 102:1730–1736.
Bartmann P, Gortner L, Pohlandt F, Jaeger H: In vitro lymphocyte functions in the presence of bovine surfactant and its phospholipid fractions. J Perinat Med 1992, 20:189–196.
Carroll JL Jr, McCoy DM, McGowan SE, Salome RG, Ryan AJ, Mallampalli RK: Pulmonary-specific expression of tumor necrosis factor-alpha alters surfactant lipid metabolism. Am J Physiol Lung Cell Mol Physiol 2002, 282:L735–42.
Whitsett JA, Budden A, Hull WM, Clark JC, O'Reilly MA: Transforming growth factor-beta inhibits surfactant protein A expression in vitro. Biochim Biophys Acta 1992, 1123:257–262.
Dulkerian SJ, Gonzales LW, Ning Y, Ballard PL: Regulation of surfactant protein D in human fetal lung. Am J Respir Cell Mol Biol 1996, 15:781–786.
Pappagianis D, The Valley Fever Vaccine Study Group: Evaluation of the protective efficacy of the killed Coccidioides immitis spherule vaccine in humans. Am Rev Respir Dis 1993, 148:656–660.