Cloning of sgk serine-threonine protein kinase from shark rectal gland – a gene induced by hypertonicity and secretagogues

Pflügers Archiv - Tập 436 - Trang 575-580 - 1998
S. Waldegger1, Petra Barth1, John N. Forrest Jr.2, Rainer Greger3, Florian Lang1
1Department of Physiology I, University of Tübingen, Gmelinstr. 5, D-72076 Tübingen, Germany, , DE
2Department of Medicine, Yale University, New Haven CT-06510, USA, , US
3Department of Physiology, University of Freiburg, D-79104 Freiburg, Germany, , DE

Tóm tắt

 Recently, the cell-volume-regulated serine-threonine protein kinase h-sgk was cloned from a human hepatoma cell line. The sgk gene was shown to be induced by cell shrinkage in many different mammalian cell lines. In this study, two highly conserved serine-threonine protein kinases, sgk-1 and sgk-2, were cloned from rectal gland tissue of the spiny dogfish (Squalus acanthias). Both kinases showed a distinct pattern of tissue specificity, with high expression levels in kidney, intestine, liver and heart. In rectal gland slices sgk-1 transcription was induced by exposure to hypertonic solution, reduction of the extracellular urea concentration, and addition of the secretagogues vasoactive intestinal polypeptide (VIP) and carbachol. The shark sgk-1 serine-threonine protein kinase may therefore provide a link between cell volume, Cl–secretion and protein phosphorylation state in shark rectal gland cells.