Michael R. Jackson1,2, LEE McCARTHY1,2, David Harding1,2, Samuel H. Wilson1,2, Michael W.H. Coughtrie1,2, Brian Burchell1,2
1Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, U.K.
2Department of Obstetrics and Gynaecology, Bristol Maternity Hospital, Bristol BS2 8EG, U.K.
Tóm tắt
A cDNA clone (HLUG 25) encoding the complete sequence of a human liver UDP-glucuronosyltransferase was isolated from a lambda gt11 human liver cDNA library. The library was screened by hybridization to a partial-length human UDP-glucuronosyltransferase cDNA (pHUDPGT1) identified from a human liver pEX cDNA expression library by using anti-UDP-glucuronosyltransferase antibodies. The authenticity of the cDNA clone was confirmed by hybrid-select translation and extensive sequence homology to rat liver UDP-glucuronosyltransferase cDNAs. The sequence of HLUG 25 cDNA was determined to be 2104 base-pairs long, including a poly(A) tail, and contains a long open reading frame. The possible site of translation initiation of this sequence is discussed with reference to a rat UDP-glucuronosyltransferase cDNA clone (RLUG 38).
