Cloning and Functional Analysis of the <i>pbr</i> Lead Resistance Determinant of <i>Ralstonia metallidurans</i> CH34

Journal of Bacteriology - Tập 183 Số 19 - Trang 5651-5658 - 2001
Brigitte Borremans1, Jon L. Hobman2, Ann Provoost1, Nigel L. Brown2, Daniël van der Lelie1
1VITO, Vlaamse Instelling voor Technologisch Onderzoek, Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium,1 and
2School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom2

Tóm tắt

ABSTRACT The lead resistance operon, pbr , of Ralstonia metallidurans (formerly Alcaligenes eutrophus ) strain CH34 is unique, as it combines functions involved in uptake, efflux, and accumulation of Pb(II). The pbr lead resistance locus contains the following structural resistance genes: (i) pbrT , which encodes a Pb(II) uptake protein; (ii) pbrA , which encodes a P-type Pb(II) efflux ATPase; (iii) pbrB , which encodes a predicted integral membrane protein of unknown function; and (iv) pbrC , which encodes a predicted prolipoprotein signal peptidase. Downstream of pbrC , the pbrD gene, encoding a Pb(II)-binding protein, was identified in a region of DNA, which was essential for functional lead sequestration. Pb(II)-dependent inducible transcription of pbrABCD from the PpbrA promoter is regulated by PbrR, which belongs to the MerR family of metal ion-sensing regulatory proteins. This is the first report of a mechanism for specific lead resistance in any bacterial genus.

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Journal of Bacteriology

Tập 183 Số 19

5651-5658

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