Clofibrate feeding increases cytoplasmic but not mitochondrial malic enzyme activity in rat kidney cortex

Cell Biochemistry and Function - Tập 7 Số 2 - Trang 97-103 - 1989
M Zelewski1, Julian Świerczyński
1Department of Biochemistry, Academic Medical School, Gdansk, Poland

Tóm tắt

AbstractAdministration of clofibrate for 21 days to rats increased the malic enzyme activity in the kidney cortex by about 80 per cent. This effect seems to be specific since the drug did not alter significantly the activity either of lactate dehydrogenase, citrate synthase or total mitochondrial protein content in this organ.The increase in activity of malic enzyme in the 13 000 g supernatant (extramitochondrial) fraction in rats treated with the drug was about 80 per cent, whereas in the pellet (mitochondrial fraction) it was about 40 per cent. The specific activity of malic enzyme in the kidney cortex cytosol from clofibrate‐treated rats was about twice that in controls. In contrast clofibrate treatment did not affect its specific activity in isolated mitochondria.Calculations showed that 0·57 and 0·53 μmoles min−1 g−1 wet tissue of mitochondrial malic enzyme was obtained in control and clofibrate‐treated rats respectively. Thus, clofibrate feeding increases the amount of cytoplasmic but not mitochondrial malic enzyme activity.

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Tài liệu tham khảo

10.1111/j.1432-1033.1971.tb01347.x

10.1016/0005-2744(72)90148-9

10.1016/0005-2744(80)90273-9

10.1016/0005-2744(80)90258-2

10.1016/0020-711X(81)90105-1

Simpson E., 1969, Advances in Enzyme Regulation, 259

10.1016/0006-2944(82)90077-1

10.1016/0143-4004(87)90020-8

10.1016/0020-711X(85)90215-0

10.1042/bj1860799

10.1159/000112748

10.1016/B978-0-12-152809-6.50012-3

Dixit A., 1983, Influence of 2‐meracptopropionyl‐glycine on DMBA – induced biochemical changes in regenerating mouse liver: II. Glucose – 6‐phosphate dehydrogenase, malic enzyme, DNA and protein, Biochem. Int., 7, 775

10.1016/0304-4165(83)90296-9

10.1016/0306-3623(86)90023-6

10.1016/0026-0495(65)90111-3

Żelewski M.andAwierczyñski J.(1987). Clofibrate feeding increase cytoplasmic but not mitochondrial malic enzyme in rat kidney cortex. Proceedings of the 28th Congress of the European Society of Toxicology – Eurotox 87′ 74 bis.

10.1016/0076-6879(67)10018-9

Lipsky N. G., 1982, Perturbation by clofibrate of mitochondrial levels in animal cells. Implications for a model of mitochondrial genesis, J. Biol. Chem., 257, 1473, 10.1016/S0021-9258(19)68218-9

10.1016/0304-4165(83)90335-5

10.1021/bi00341a044

Tepperman H. M., 1964, Patterns of dietetary and hormonal induction of certain NADP – linked liver enzymes, Am. J. Physiol., 206, 357, 10.1152/ajplegacy.1964.206.2.357

Lee Y. P., 1965, Influence of thyroid hormones on L‐α‐glycerophosphate dehydrogenase and other dehydrogenases in various organ of the rat, J. Biol. Chem., 240, 1427, 10.1016/S0021-9258(18)97593-9

10.1016/0021-9150(79)90146-1

10.1152/physrev.1983.63.4.1420

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Cell Biochemistry and Function

Tập 7 Số 2

97-103

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