Citrulline induces fatty acid release selectively in visceral adipose tissue from old rats

Molecular Nutrition and Food Research - Tập 58 Số 9 - Trang 1765-1775 - 2014
Nolwenn Joffin1,2, Anne‐Marie Jaubert3,1, Sylvie Durant1,2, Jean Bastin1,2, Jean‐Pascal De Bandt4,5,2, Luc Cynober4,5,2, Christophe Moinard4,2, Claude Forest1,2, Philippe Noirez6,7,2
1Institut National de la Santé et de la Recherche Médicale UMR‐S 1124 Faculté des Sciences Fondamentales et Biomédicales Pharmacologie Toxicologie et Signalisation Cellulaire Paris France
2Université Paris Descartes, Sorbonne Paris Cité, France
3Département de Biochimie et de Biologie Moléculaire Faculté de Médecine Paris‐Ile de France‐Ouest Université de Versailles Saint‐Quentin en Yvelines Versailles France
4Laboratoire de Biologie de la Nutrition Faculté des Sciences Pharmaceutiques et Biologiques Paris France
5Service de Biochimie, Hôpital Cochin, Assistance Publique Hôpitaux de Paris, Paris, France;
6Institut de Recherche Biomédicale et d’Epidémiologie du Sport Paris France
7UFR des Sciences et Techniques des Activités Physiques et Sportives Paris France

Tóm tắt

ScopeDuring aging, increased visceral adipose tissue (AT) mass may result in impaired metabolic status. A citrulline (CIT)‐supplemented diet reduces AT mass in old rats. We hypothesized that CIT could directly affect fatty acid (FA) metabolism in retroperitoneal AT.Methods and resultsA 24‐h exposure of AT explants from old (25 months) rats to 2.5 mM CIT induced a 50% rise in glycerol and FA release, which was not observed in explants from young (2 months) animals. The phosphorylated form of hormone‐sensitive lipase, a key lipolytic enzyme, was 1.5‐fold higher in CIT‐treated explants from old and young rats, whereas glyceroneogenesis, that provides glycerol‐3P requested for FA re‐esterification, and its key enzyme phosphoenolpyruvate carboxykinase, were down‐regulated 40–70%. Specifically in young rats, beta‐oxidation capacity and gene expressions of carnitine palmitoyl transferase 1‐b and very long chain acyl‐CoA dehydrogenase were strongly up‐regulated by CIT. In contrast, in old rats, while glyceroneogenesis was lower, beta‐oxidation was not affected, enabling increased FA release.ConclusionHence, in visceral AT, CIT exerts a specific induction of the beta‐oxidation capacity in young rats and a selective stimulation of FA release in old rats, therefore providing a direct mechanism of CIT action to reduce AT mass.

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Molecular Nutrition and Food Research

Tập 58 Số 9

1765-1775

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