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Các exosome huyết thanh tuần hoàn i-tRF-AspGTC và tRF-1-SerCGA như các chỉ thị chẩn đoán cho ung thư phổi không phải tế bào nhỏ
Tóm tắt
tRF-RNA—một đại diện của RNA không mã hóa (ncRNA)—là một tiền chất hoặc mảnh của tRNA trưởng thành và đóng vai trò điều hòa quan trọng trong quá trình hình thành và phát triển của ung thư. Hiện tại, có rất ít nghiên cứu về tRF-RNA như một dấu hiệu chẩn đoán trong ung thư, đặc biệt là ung thư phổi không phải tế bào nhỏ (NSCLC) từ exosome huyết thanh. Exosome huyết thanh đã được chiết xuất thành công từ huyết thanh; hình thái vật lý của chúng được ghi lại bằng kính hiển vi điện tử truyền qua (TEM); việc phát hiện kích thước hạt phù hợp đã được thực hiện bằng cách sử dụng qNano; việc gán bề mặt đã được xác minh thông qua phân tích western blotting. Exosome huyết thanh i-tRF-AspGTC và tRF-1-SerCGA đã được chọn thông qua vi mảng gen, và qPCR đã được sử dụng để xác thực tầm quan trọng của chúng ở 242 bệnh nhân và 201 cá nhân khỏe mạnh. Diện tích dưới đường cong (AUC) đã được sử dụng để đánh giá các chỉ số chẩn đoán của ung thư phổi không phải tế bào nhỏ (NSCLC). So với 201 cá nhân khỏe mạnh, i-tRF-AspGTC và tRF-1-SerCGA đã giảm đáng kể ở 242 bệnh nhân NSCLC và 95 bệnh nhân ở giai đoạn đầu. Đối với tRF-AspGTC và tRF-1-SerCGA, tỷ lệ hiệu suất chẩn đoán dự đoán của AUC lần lượt là 0.690 và 0.680, trong khi tỷ lệ hiệu suất chẩn đoán sớm là 0.656 và 0.688, tương ứng. Kết quả của việc chẩn đoán kết hợp với CEA và CYFRA21-1 là 0.928, và hiệu suất chẩn đoán sớm là 0.843, đây là một yếu tố dự đoán sinh học rất cao cho NSCLC. Biểu hiện của các exosome huyết thanh i-tRF-AspGTC và tRF-1-SerCGA đã giảm đáng kể ở bệnh nhân NSCLC. Những exosome này có thể được sử dụng như các chỉ số dự đoán cho chẩn đoán hoặc chẩn đoán sớm ung thư phổi không phải tế bào nhỏ (NSCLC).
Từ khóa
#tRF-RNA #ung thư phổi không phải tế bào nhỏ #exosome huyết thanh #chỉ thị chẩn đoán #qPCR #khám sàng lọcTài liệu tham khảo
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