Chromosome aberration and sister chromatid exchange tests in chinese hamster ovary cells in vitro: II. Results with 20 chemicals
Tóm tắt
Twenty chemicals were tested for their ability to induce sister chromatid exchanges (SCEs) and chromosomal aberrations (ABs) in cultured Chinese hamster ovary cells (CHO). These chemicals were tested with and without an added metabolic activation system (rat liver S9 fraction). Four chemicals were negative in both assays, 1 induced ABs only, and 15 were positive for SCEs; 6 of these 15 also induced ABs.
The effect of cell harvest time on the ability to detect the induction of chromosomal aberrations was examined for six chemicals. Five of these had caused at least one of the following: cell cycle delay, aberrations observed in first division metaphase cells in the SCE assay, or a weak response in the standard AB assay (10–12‐hr growth period). Three chemicals, chlorinated tri‐sodium phosphate, 1, 2‐dibromo‐3‐chloropro‐pane, and tetrakis(hydroxymethyl)phosphonium chloride, were positive using both the standard and extended harvest times. N‐Nitrosodimethyl‐amine and diphenhydramine HCI were only positive using an extended harvest time, and malo‐naldehyde was negative using both standard and extended harvest times.
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Tài liệu tham khảo
Cantu DS, 1985, Fragile (X) expression induced by FUdR is transient and inversely related to levels of thymidylate synthetase activity, Am J Hum Genet, 37, 947
DeanBJ(1981): Activity of 27 coded compounds in the RL1 chromosome assay. In de Serres F Ashby J (eds): “Evaluation of Short‐Term Tests for Carcinogens: Report of the International Collaborative Program.” Prog Mutat Res 1:570–579.
Dougherty RW, 1978, Failure of 2, 4‐dinitrotoluene to induce dominant lethal mutations in the rat, The Pharmacologist, 20, 155
Glover TW, 1981, FUdR induction of the X chromosome fragile site: Evidence for the mechanism of folic acid and thymidine inhibition, Am J Hum Genet, 33, 234
Ishidate M, 1981, Chromosomal aberration tests in vitro as a primary screening tool for environmental mutagens and/or carcinogens, Gann Monogr Cancer Res, 27, 95
Latt SA, 1980, Advances in Human Genetics, 267
Margolin BH, 1985, A simple experimental method to probe the resolving power of an in vitro toxicological assay, Environ Mutagen, 7, 48
Probst GS, 1981, Genotoxicity of some antihistamines in a battery of short‐term tests, Environ Mutagen, 3, 326
Sen P, 1985, Induction of chromosomal damage in Chinese hamster ovary cells by soluble and particulate nickel compounds: Preferential fragmentation of the heterochromatic long arm of the X‐chromosome by carcinogenic crystalline NiS particles, Cancer Res, 45, 2320
Sutherland GR, 1979, Heritable fragile sites on human chromosomes. I. Factors affecting expression in lymphocyte culture, Am J Hum Genet, 31, 125
Tomkins D, 1980, Testing of pesticides for induction of sister chromatid exchange in Chinese hamster ovary cells, Can J Genet Cytol, 22, 681