Chemotherapy in experimental brain tumor, part 1: In vitro colorimetric MTT assay
Tóm tắt
This study concerns the use of the MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] colorimetric assay to evaluate the chemosensitivity of the C6 rat glioma cell line to a panel of twelve chemotherapeutic agents. In a previous study of in vitro chemosensitivity of human glioma cell lines, the present authors found a range of sensitivities of the respective cell lines to a panel of chemotherapeutic agents [1]. We then devised an experimental strategy to begin an in vivo evaluation of the correlation between in vitro chemosensitivity and clinical response in an in vivo animal model, such as the model employing the C6 rat glioma cell line. As a step towards utilizing the C6 rat glioma in vivo model, we carried out the present study (Part 1) to determine the correspondence between chemosensitivity to human glioma cell lines and the rat C6 glioma cell line. If a correspondence were to be found, this would enable experimental use of the C6 tumor model for in vivo testing of chemotherapeutic agents. As reported in this paper (Part 1), a correspondence was found, suggesting that the C6 rat glioma represents a suitabe model of human glioma for chemotherapeutic studies. This finding served as a basis for proceeding with an in vivo study of chemotherapeutic efficacy which is the subject of a companion report [2].
Tài liệu tham khảo
Jordan JJ, Hand CM, Markowitz RS, Black P: Test for chemotherapeutic sensitivity of cerebral gliomas: use of colorimetric MTT assay. J Neuro Oncol 14: 19–35, 1992
Black P, Hand CM, Vender JR, Lehmann J, Finkelstein SD: Chemotherapy in experimental brain tumor, part 2: pretreatment with leukotriene C4 prolongs survival. J Neuro Oncol 36: 7–19, 1998
Hongo T, Fujii Y, Igarashi Y: An in vitro chemosensitivity test for the screening of anti-cancer drugs in childhood leukemia. Cancer 65: 1263–1271, 1990
Metcalfe SA: A review of methods for estimating clinically achievable antitumor drug levels and their association with studies in vitro. In: Dendy PP, Hill BT (eds) Human Tumour Drug Sensitivity Testing In Vitro. Academic Press, London 213–224, 1983
Nikkhah G, Tonn JC, Hoffman O, Kraemer HP, Darling JL, Schonmayr R: The MTT assay for chemosensitivity testing of human tumors of the central nervous system: part 1: evaluation of test-specific variables. J Neuro-Oncol 13: 1–11, 1992
Nikkhah G, Tonn JC, Hoffman O, Kraemer HP, Darling JL, Schachenmayr W, Schonmayr R: The MTT assay for chemosensitivity testing of human tumors of the central nervous system: part 2: evaluation of patient-and drug-specific variables. J Neuro-Oncol 13: 13–24, 1992
Von Hoff DD, Clark GM, Stogdill BJ, Sarodsky MF, O'Brien MT, Casper JT, Mattox DE, Page CP, Cruz AB, Sandbach JF: Prospective clinical trial of a human tumorcloning system. Cancer Res 43: 1926–1931, 1983
Kirkpatrick DL, Duke M, Goh TS: Chemosensitivity testing of fresh human leukemia cells using both a dye exclusion assay and a tetrazolium dye (MTT) assay. Leukemia Res 14 (5): 459–466, 1990
Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB: Evaluation of a tetrazolium-based semi-automatic colorimetric assay. Cancer Res 47: 943–946, 1987
Carmichael J, Mitchell JB, DeGraff WG, Gamson J, Gazdar AF, Johnson BE, Glatstein E, Minna JD: Chemosensitivity testing of human lung cancer cell lines using the MTT assay. Br J Cancer 57(6): 540–547, 1988
Twentyman PR, Fox NE, Rees JKH: Chemosensitivity testing of fresh leukaemia cells using the MTT colorimetric assay. Br J Haematol 71: 19–24, 1989
Durkin WJ, Ghanta VK, Hiramoto RN: Results obtained using a vital dye exclusion assay and clinical correlations. In: Dendy PP, Hill BT (eds) Human Tumor Drug Sensitivity Testing In Vitro. Academic Press, London 259–268, 1983
Green LM, Reade JL, Ware CF: Rapid colorimetric assay for cell viability: application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods 70: 257–268, 1984
Kornblith PL, Szypko PE: Variations in response of human brain tumors to BCNU in vitro. J Neurosurg 48: 580–586, 1978
Sargeant JM, Taylor CG: Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeloid leukemia. Br J Cancer 60: 206–210, 1989