Chemotaxis of the peritoneal cells and the detection of a chemo‐attractant in the effluent from peritoneal dialysis patients

European Journal of Clinical Investigation - Tập 20 Số s1 - Trang 555-562 - 1990
H J Bos1, D. M. Boorsma2, Cornelis W. Tuk1, J. C. de Veld1, A. J. C. VAN DER MUYSENBERG1, T J Helmerhorst3, Dirk G. Struijk4, H van Bronswijk5, R.H.J. Beelen6
1*Departments of Cell Biology, Universal Hospital, Free University, Amsterdam
2d̀Dermatology, Universal Hospital, Free University, Amsterdam
3‡Obstetrics and Gynaecology, Universal Hospital, Free University, Amsterdam
4**Department of Nephrology, University Hospital, Academic Medical Centre, Amsterdam, The Netherlands
5§Internal Medicine, Division of Nephrology, Universal Hospital, Free University, Amsterdam
6¶Haematology, Medical Faculty and Universal Hospital, Free University, Amsterdam

Tóm tắt

Abstract. The migration of peritoneal cells from 25 continuous ambulatory peritoneal dialysis patients and eight healthy women undergoing laparoscopy were studied. Peritoneal cells of continuous ambulatory peritoneal dialysis patients migrated to commonly used chemoattractants, like N‐formyl‐methionyl‐leu‐cyl‐phenyl‐alanine‐methyl‐ester and casein, but they also migrated to high concentrations of recombinant interleukin‐1 α and to endotoxin (lipopolysaccharide). In the peritoneal effluent from continuous ambulatory peritoneal dialysis patients a rather heat stable chemo‐attractant was found with a molecular weight of 40–200 kDa with an optimal activity at approximately 125 kDa. The chemoattractant is a protein and is not complement factor 5a or interleukin‐1 and was only found in peritoneal effluent from continuous ambulatory peritoneal dialysis patients, but not in peritoneal fluid from healthy women undergoing laparoscopy. Therefore, peritoneal dialysis might induce the generation of a chemoattractant. The optimal chemotactic response of peritoneal cells from continuous ambulatory peritoneal dialysis patients to N‐formyl‐methionyl‐leucyl‐phenyl‐alanine‐methyl‐ester in medium could be enhanced by replacing the medium by peritoneal effluent. So the chemotaxis of peritoneal cells to the factor in the peritoneal effluent is caused by another mechanism, which might involve different cell surface receptor populations, than the chemotactic response to N‐formyl‐methionyl‐leucyl‐phenyl‐alanine‐methyl‐ester.

Từ khóa


Tài liệu tham khảo

Kurz SB, 1983, Continuous ambulatory peritoneal dialysis. Three years experience at the Mayo Clinic, Mayo Clin Proc, 58, 633

Peterson PK, 1985, Current Clinical Topics in Infectious Diseases, 239

Bos HJ, 1988, Peritoneal macrophages in short dwell time effluent show diminished phagocytosis, Perit Dial Int, 8, 199, 10.1177/089686088800800305

10.1038/ki.1984.209

Rubin J., 1983, Host defence mechanisms in continuous ambulatory peritoneal dialysis, Clin Nephrol, 20, 104

10.1002/jlb.43.2.172

10.1016/S0002-9378(16)32537-6

10.1016/S0002-9378(84)80037-X

10.1016/S0015-0282(16)45567-6

10.1002/jcp.1041150110

10.1084/jem.136.1.128

Falk W., 1980, A 48‐well micro chemotaxis assembly for rapid and accurate measurement of leucocyte migration, J Immunol Meth, 33, 239, 10.1016/S0022-1759(80)80014-7

10.1016/0022-1759(80)90179-9

10.1084/jem.137.2.387

Matzncr Y., 1984, A C5a inhibitor in peritoneal fluid, J Lab Clin Med, 103, 227

10.1002/jcp.1041180102

10.1038/ki.1984.167

10.1038/ki.1984.177

10.1002/jlb.39.4.457

Dasgupta MK, 1986, Advances in Continuous Ambulatory Peritoneal Dialysis, 102

10.1146/annurev.iy.02.040184.002333

Morrison DC, 1978, The effect of bacterial endotoxinson host mediation systems, Am J Pathol, 93, 526

10.1084/jem.127.4.693

Allison AC., 1978, Macrophage activation and nonspecific immunity, Int Rev Exp Pathol, 18, 303

10.1016/S0065-2776(08)60802-0

10.1016/S0065-2776(08)60919-0

10.1038/ki.1989.155

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Thông tin xuất bản

European Journal of Clinical Investigation

Tập 20 Số s1

555-562

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