Chemometric evaluation of hypericin and related phytochemicals in 17 <i>in vitro</i> cultured <i>Hypericum</i> species, hairy root cultures and hairy root-derived transgenic plants

Journal of Pharmacy and Pharmacology - Tập 71 Số 1 - Trang 46-57 - 2019
Katarína Nigútová1, Souvik Kusari2, Selahaddin Sezgin2, Linda Petijová1, Jana Henzelyová1, Miroslava Bálintová1, Michael Spiteller2, Eva Čellárová1
1Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Košice, Slovakia
2Institute of Environmental Research (INFU), Department of Chemistry and Chemical Biology, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Dortmund, Germany

Tóm tắt

Abstract Objectives The objective of this study was to ascertain the presence and correlations among eight important secondary metabolites viz. hypericin, pseudohypericin, emodin, hyperforin, rutin, hyperoside, quercetin and quercitrin in different organs of 17 in vitro cultured Hypericum species, along with H. tomentosum and H. tetrapterum hairy root cultures, and hairy root-derived transgenic plants of H. tomentosum. Methods Samples were extracted and analysed by LC-MS. The LC-MS data were subjected to chemometric evaluations for metabolite profiling and correlating the phytochemical compositions in different samples. Key findings Hypericin, pseudohypericin and their proposed precursor emodin were detected in various levels in the leaves of eight Hypericum species. The highest content of hypericins and emodin was found in H. tetrapterum, which contains the studied secondary metabolites in all plant organs. A significant positive correlation between hypericins and emodin was observed both by principal component analysis (PCA) and multidimensional scaling (MDS), indicating the role of emodin as a possible precursor in the biosynthetic pathway of hypericins. Flavonoids were found in all tested plant organs except roots of H. pulchrum. The hairy roots lacked hypericin, pseudohypericin, emodin, hyperforin and rutin. However, the hairy root-derived transgenic plants showed a significant increase in flavonoids. Conclusions This study broadens knowledge about the phytochemical composition of selected in vitro cultured Hypericum species, compared to that of hairy root cultures and hairy root-derived transgenic plants.

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Thông tin xuất bản

Journal of Pharmacy and Pharmacology

Tập 71 Số 1

46-57

Thông tin tác giả