Chemolithotrophic acetogenic H2/CO2 utilization in Italian rice field soil

ISME Journal - Tập 5 Số 9 - Trang 1526-1539 - 2011
Fanghua Liu1, Ralf Conrad1
1Department of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany

Tóm tắt

Abstract Acetate oxidation in Italian rice field at 50 °C is achieved by uncultured syntrophic acetate oxidizers. As these bacteria are closely related to acetogens, they may potentially also be able to synthesize acetate chemolithoautotrophically. Labeling studies using exogenous H2 (80%) and 13CO2 (20%), indeed demonstrated production of acetate as almost exclusive primary product not only at 50 °C but also at 15 °C. Small amounts of formate, propionate and butyrate were also produced from 13CO2. At 50 °C, acetate was first produced but later on consumed with formation of CH4. Acetate was also produced in the absence of exogenous H2 albeit to lower concentrations. The acetogenic bacteria and methanogenic archaea were targeted by stable isotope probing of ribosomal RNA (rRNA). Using quantitative PCR, 13C-labeled bacterial rRNA was detected after 20 days of incubation with 13CO2. In the heavy fractions at 15 °C, terminal restriction fragment length polymorphism, cloning and sequencing of 16S rRNA showed that Clostridium cluster I and uncultured Peptococcaceae assimilated 13CO2 in the presence and absence of exogenous H2, respectively. A similar experiment showed that Thermoanaerobacteriaceae and Acidobacteriaceae were dominant in the 13C treatment at 50 °C. Assimilation of 13CO2 into archaeal rRNA was detected at 15 °C and 50 °C, mostly into Methanocellales, Methanobacteriales and rice cluster III. Acetoclastic methanogenic archaea were not detected. The above results showed the potential for acetogenesis in the presence and absence of exogenous H2 at both 15 °C and 50 °C. However, syntrophic acetate oxidizers seemed to be only active at 50 °C, while other bacterial groups were active at 15 °C.

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ISME Journal

Tập 5 Số 9

1526-1539

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