Chemisch definiert – ein zellbasierter Zytotoxizitätsassay ohne fötales Kälberserum
Tóm tắt
Tests for cytotoxicity–respectively biocompatibility–are performed e. g. for biological evaluation of medical devices or during the development of microphysiometric systems. Here, often cell culture methods which involve the use of fetal bovine serum (FBS) are employed. However, using FBS raises scientific and ethical concerns. In the presented work a protocol for a chemically defined test for cytotoxicity is described.
Tài liệu tham khảo
citation_journal_title=Toxicol In Vitro; citation_title=Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods; citation_author=J Valk, D Brunner, K Smet; citation_volume=24; citation_publication_date=2010; citation_pages=1053-1063; citation_doi=10.1016/j.tiv.2010.03.016; citation_id=CR1
citation_title=Fetal bovine serum (FBS): past–present–future; citation_publication_date=2017; citation_id=CR2; citation_author=J Valk
citation_title=Zell-und Gewebekultur; citation_publication_date=2013; citation_id=CR3; citation_author=G Gstraunthaler; citation_author=T Lindl; citation_publisher=Springer Spektrum