Chemiluminescence Immunoassay for Progesterone in Plasma Incorporating Acridinium Ester Labelled Antigen

Annals of Clinical Biochemistry - Tập 25 Số 1 - Trang 27-34 - 1988
Stuart Miller1, Michael S. Scott Morton1, A. Turkes1
1Tenovus Institute for Cancer Research, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XX, UK

Tóm tắt

A sensitive, solid-phase chemiluminescence immunoassay suitable for determining progesterone concentrations in plasma has been developed. The solid-phase antiserum was prepared by coupling a monoclonal progesterone-antibody, raised against a progesterone-11α-hemisuccinyl/bovine serum albumin conjugate, to cyanogen bromide activated cellulose. An 11α-progesteryl-2-carboxymethyltyramine-4-(10-methyl)-acridinium-9-carboxylate conjugate was used as the chemiluminescent label. The assay had a lower limit of sensitivity of 3 pg/assay tube and satisfied accepted validation criteria. Progesterone concentrations determined by chemiluminescence assay were in good agreement not only with a radioimmunoassay in routine use but also with a gas chromatography-mass spectrometry procedure.

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Thông tin
Thông tin xuất bản

Annals of Clinical Biochemistry

Tập 25 Số 1

27-34

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