Yuji Naito1, Misty Williams-Fritze2, Daniel R. Duncan2, Spencer N. Church2, Narutoshi Hibino2, Joseph A. Madri2, Jay D. Humphrey3, Toshiharu Shinoka2, Christopher K. Breuer2
1Department of Surgery, Yale University School of Medicine, New Haven, Conn., USA
2Yale University
3Yale School of Engineering and Applied Science
Tóm tắt
<i>Background:</i> The extracellular matrix (ECM) is a critical determinant of neovessel integrity. <i>Materials and Methods:</i> Thirty-six (polyglycolic acid + polycaprolactone and poly lactic acid) tissue-engineered vascular grafts seeded with syngeneic bone marrow mononuclear cells were implanted as inferior vena cava interposition grafts in C57BL/6 mice. Specimens were characterized using immunohistochemical staining and qPCR for representative ECM components in addition to matrix metalloproteinases (MMPs). Total collagen, elastin, and glycosaminoglycan (GAG) contents were determined. MMP activity was measured using zymography. <i>Results:</i> Collagen production on histology demonstrated an initial increase in type III at 1 week followed by type I production at 2 weeks and type IV at 4 weeks. Gene expression of both type I and type III peaked at 2 weeks, whereas type IV continued to increase over the 4-week period. Histology demonstrated fibrillin-1 deposition at 1 week followed by elastin production at 4 weeks. Elastin gene expression significantly increased at 4 weeks, whereas fibrillin-1 decreased at 4 weeks. GAG demonstrated abundant production at each time point on histology. Gene expression of decorin significantly increased at 4 weeks, whereas versican decreased over time. Biochemical analysis showed that total collagen production was greatest at 2 weeks, and there was a significant increase in elastin and GAG production at 4 weeks. Histological characterization of MMPs showed abundant production of MMP-2 at each time point, while MMP-9 decreased over the 4-week period. Gene expression of MMP-2 significantly increased at 4 weeks, whereas MMP-9 significantly decreased at 4 weeks. <i>Conclusions:</i> ECM production during neovessel formation is characterized by early ECM deposition followed by extensive remodeling.
