Characterization of sinusoidal endothelial cells of the liver and bone marrow using an intravital lectin injection method
Tóm tắt
The vascular endothelia express a variety of structural and biological phenotypes. We used an intravital injection method of plant derived lectins (Lycopersicon esculentum lectin (LEL), Ricinus communis Agglutinin-I (RCA-I), Ulex europaeus Agglutinin-I (UEA-I) and Concanavalin A (ConA)) to elucidate the morphology and function of the sinusoidal endothelium of the liver and bone marrow. All four lectins stained the sinusoidal endothelia of the liver and bone marrow in a patchy granular pattern which differed from the uniform and smooth staining pattern of non-sinusoidal vessels in other organs. By transmission electron microscopy, the granular pattern was identified as internalization of these lectins and subsequent accumulation within the endothelial cells, suggesting their active endocytosis. The endocytosis of these lectins emphasizes the fact that sinusoidal endothelial cells of the liver and bone marrow belong to the reticuloendothelial system (RES), a cell system characterized by internalization of foreign material. We introduce this intravital lectin injection as a useful technique to discriminate sinusoidal endothelial of the liver and bone marrow from other vascular endothelia.
Tài liệu tham khảo
Ashcoff L (1924) Das reticulo-endotheliale system. Erg Inn Med Kinderheilkd 26:1–118
Belloni PN, Nicolson GL (1988) Differential expression of cell surface glycoproteins on various organ-derived microvascular endothelia and endothelial cell cultures. J Cell Physiol 136(3):398–410
Debbage PL, Griebel J et al (1998) Lectin intravital perfusion studies in tumor-bearing mice: micrometer-resolution, wide-area mapping of microvascular labeling, distinguishing efficiently and inefficiently perfused microregions in the tumor. J Histochem Cytochem 46(5):627–640
Debbage PL, Solder E et al (2001) Intravital lectin perfusion analysis of vascular permeability in human micro- and macro- blood vessels. Histochem Cell Biol 116(4):349–359
De Bruyn PP, Michelson S (1981) An anionic material at the advancing front of blood cells entering the bone marrow circulation. Blood 57(1):152–156
De Bruyn PP, Michelson S et al (1975) Endocytosis, transfer tubules, and lysosomal activity in myeloid sinusoidal endothelium. J Ultrastruct Res 53(2):133–151
Draenert K, Draenert Y (1980) The vascular system of bone marrow. Scan Electron Microsc 4:113–122
Fawcett DW (1994) A textbook of histology. Chapman & Hall, New York
Hillyer P, Male D (2005) Expression of chemokines on the surface of different human endothelia. Immunol Cell Biol 83(4):375
Kuzu I, Bicknell R et al (1993) Expression of adhesion molecules on the endothelium of normal tissue vessels and vascular tumors. Lab Invest 69(3):322–328
Magnusson S, Berg T (1993) Endocytosis of ricin by rat liver cells in vivo and in vitro is mainly mediated by mannose receptors on sinusoidal endothelial cells. Biochem J 291(Pt 3):749–755
Mellman I (1996) Endocytosis and molecular sorting. Annu Rev Cell Dev Biol 12:575–625
Morikawa S, Baluk P et al (2002) Abnormalities in pericytes on blood vessels and endothelial sprouts in tumors. Am J Pathol 160(3):985–1000
Mukherjee S, Ghosh RN et al (1997) Endocytosis. Physiol Rev 77(3):759–803
Petri B, Bixel MG (2006) Molecular events during leukocyte diapedesis. FEBS J 273(19):4399–4407
Risau W (1995) Differentiation of endothelium. FASEB J 9(10):926–933
Sandvig K, van Deurs B (2005) Delivery into cells: lessons learned from plant and bacterial toxins. Gene Ther 12(11):865
Sandvig K, Spilsberg B et al (2004) Pathways followed by protein toxins into cells. Int J Med Microbiol 293(7–8):483–490
Schenkel AR, Chew TW et al (2004) Platelet endothelial cell adhesion molecule deficiency or blockade significantly reduces leukocyte emigration in a majority of mouse strains. J Immunol 173(10):6403–6408
Sharon N, Lis H (1990) Legume lectins—a large family of homologous proteins. FASEB J 4(14):3198–3208
Simionescu M, Simionescu N (1986) Functions of the endothelial cell surface. Annu Rev Physiol 48:279–293
Turner RR, Beckstead JH et al (1987) Endothelial cell phenotypic diversity. In situ demonstration of immunologic and enzymatic heterogeneity that correlates with specific morphologic subtypes. Am J Clin Pathol 87(5):569–575
van Deurs B, Tonnessen TI et al (1986) Routing of internalized ricin and ricin conjugates to the Golgi complex. J Cell Biol 102(1):37–47
Vorbrodt AW, Dobrogowska DH et al (1986) Ultrastructural localization of lectin receptors on the luminal and abluminal aspects of brain micro-blood vessels. J Histochem Cytochem 34(2):251–261
Weiss L (1988). Cell and tissue biology. Urban & Schwarzenberg, Inc., Baltimore