Characterization of alphasatellites associated with monopartite begomovirus/betasatellite complexes in Yunnan, China

Virology Journal - Tập 7 Số 1 - 2010
Yan Xie1, Peili Wu1, Pei Liu1, Huanran Gong1, Xueping Zhou1
1State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China

Tóm tắt

Abstract Background Alphasatellites are single-stranded molecules that are associated with monopartite begomovirus/betasatellite complexes. Results Alphasatellites were identified in begomovirus-infected plant samples in Yunnan, China. All samples that contained alphasatellites also contained betasatellites, but only some samples that contained betasatellites contained alphasatellites. Thirty-three alphasatellites were sequenced, and they ranged from 1360 to 1376 nucleotides. All alphasatellites contain 3 conserved features: a single open reading frame (Rep), a conserved hairpin structure, and an adenine-rich (A-rich) region. On the basis of the phylogenetic tree of the complete nucleotide sequences, the alphasatellites were divided into 3 types with one exception. Type 1 was associated with Tomato yellow leaf curl China virus (TYLCCNV)/Tomato yellow leaf curl China betasatellite (TYLCCNB) complex. Type 2 was associated with Tobacco curly shoot virus (TbCSV)/Tobacco curly shoot betasatellite (TbCSB) complex. Type 3 was associated with TbCSV/Ageratum yellow vein betasatellite (AYVB) complex. Within each type, nucleotide sequence identity ranged from 83.4 to 99.7%, while 63.4-81.3% identity was found between types. Mixed infections of alphasatellites associated with begomovirus/betasatellite complexes were documented. Conclusions Our results validate that alphasatellites are only associated with begomovirus/betasatellite complexes. Thirty-three sequenced alphasatellites isolated from Yunnan Province, China were divided into 3 types--each associated with a specific begomovirus/betasatellite complex. Mix-infections of alphasatellite molecules may not be unusual.

Từ khóa


Tài liệu tham khảo

Harrison B, Robinson D: Natural genomic and antigenic variation in whitefly-transmitted geminiviruses (Begomoviruses). Annu Rev Phytopathol 1999, 37: 369-398. 10.1146/annurev.phyto.37.1.369

Moffat AS: Plant pathology: geminiviruses emerge as serious crop threat. Science 1999, 286: 1835. 10.1126/science.286.5446.1835

Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA: Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses. London: Elsevier Academic Press; 2005.

Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, Malik KA, Markham PG: Identification of DNA components required for induction of cotton leaf curl disease. Virology 2001, 285: 234-243. 10.1006/viro.2001.0949

Cui X, Tao X, Xie Y, Fauquet CM, Zhou X: A DNAβ associated with Tomato yellow leaf curl China virus is required for symptom induction. J Virol 2004, 78: 13966-13974. 10.1128/JVI.78.24.13966-13974.2004

Jose J, Usha R: Bhendi yellow vein mosaic disease in India is caused by association of a DNAβ satellite with a begomovirus. Virology 2003, 305: 310-317. 10.1006/viro.2002.1768

Li Z, Xie Y, Zhou X: Tobacco curly shoot virus DNAβ is not necessary for infection but intensifies symptoms in a host-dependent manner. Phytopathol 2005, 95: 902-908. 10.1094/PHYTO-95-0902

Saunders K, Bedford ID, Briddon RW, Markham PG, Wong SM, Stanley J: A unique virus complex causes Ageratum yellow vein disease. Proc Natl Acad Sci USA 2000, 97: 6890-6895. 10.1073/pnas.97.12.6890

Zhou X, Xie Y, Tao X, Zhang Z, Li Z, Fauquet CM: Characterization of DNAβ associated with begomoviruses in China and evidence for co-evolution with their cognate viral DNA-A. J Gen Virol 2003, 84: 237-247. 10.1099/vir.0.18608-0

Mansoor S, Briddon RW, Zafar Y, Stanley J: Geminivirus disease complexes: an emerging threat. Trends Plant Sci 2003, 8: 128-134. 10.1016/S1360-1385(03)00007-4

Briddon RW, Bull SE, Amin I, Mansoor S, Bedford ID, Rishi N, Siwatch SS, Zafar Y, Abdel-Salam AM, Markham PG: Diversity of DNA 1: a satellite-like molecule associated with monopartite begomovirus-DNA beta complexes. Virology 2004, 324: 462-474. 10.1016/j.virol.2004.03.041

Mansoor S, Khan SH, Bashir A, Saeed M, Zafar Y, Malik KA, Briddon R, Stanley J, Markham PG: Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan. Virology 1999, 259: 190-199. 10.1006/viro.1999.9766

Saunders K, Stanley J: A nanovirus-like DNA component associated with yellow vein disease of Ageratum conyzoides: evidence for interfamilial recombination between plant DNA viruses. Virology 1999, 264: 142-152. 10.1006/viro.1999.9948

Wu PJ, Zhou XP: Interaction between a nanovirus-like component and the Tobacco curly shoot virus/satellite complex. Acta Biochim Biophys Sin (Shanghai) 2005, 37: 25-31. 10.1093/abbs/37.1.25

Tiendrébéogo F, Lefeuvre P, Hoareau M, Villemot J, Konaté G, Traoré AS, Barro N, Traoré VS, Reynaud B, Traoré O, Lett JM: Molecular diversity of Cotton leaf curl Gezira virus isolates and their satellite DNAs associated with Okra leaf curl disease in Burkina Faso. Virol J 2010.

Li ZH, Zhou XP, Zhang X, Xie Y: Molecular characterization of tomato-infecting begomoviruses in Yunnan, China. Arch Virol 2004, 149: 1721-1732.

Jiang T, Zhou XP: First report of Malvastrum yellow vein virus infecting Ageratum conyzoides . Plant Pathol 2004, 53: 799-799. 10.1111/j.1365-3059.2004.01098.x

Mugiira RB, Liu SS, Zhou X: Tomato yellow leaf curl virus and Tomato leaf curl Taiwan virus invade south-east coast of China. J Phytopathol 2008, 156: 217-221. 10.1111/j.1439-0434.2007.01345.x

Xie Y, Jiang T, Zhou XP: Agroinoculation shows Tobacco leaf curl Yunnan virus is a monopartite begomovirus. Eur J Plant Pathol 2006, 115: 369-375. 10.1007/s10658-006-9021-8

Xie Y, Zhou XP: Molecular characterization of squash leaf curl Yunnan virus, a new begomovirus and evidence for recombination. Arch Virol 2003, 148: 2047-2054. 10.1007/s00705-003-0153-2

Xiong Q, Fan S, Wu J, Zhou X: Ageratum yellow vein China virus is a distinct begomovirus species associated with a DNAβ molecule. Phytopathol 2007, 97: 405-411. 10.1094/PHYTO-97-4-0405

Jiang T, Liu P, Liao BL, Wu JX, Huang CJ: Malvastrum yellow vein Yunnan virus is a monopartite begomovirus. Acta Virol 2010, 54: 21-26. 10.4149/av_2010_01_21

Xie Y, Wu PJ, Tao XR, Zhou XP: Identification of a nanovirus-like DNA molecule associated with Tobacco curly shoot virus isolates containing satellite DNA. Prog Natl Sci 2004, 14: 689-693. 10.1080/10020070412331344161

Qing L, Zhou XP: Trans-replication of, and competition between, DNA beta satellites in plants inoculated with Tomato yellow leaf curl China virus and Tobacco curly shoot virus. Phytopathology 2009, 99: 716-720. 10.1094/PHYTO-99-6-0716

Kon T, Rojas MR, Abdourhamane IK, Gilbertson RL: Roles and interactions of begomoviruses and satellite DNAs associated with Okra leaf curl disease in Mali, West Africa. J Gen Virol 2009, 90: 1001-1013. 10.1099/vir.0.008102-0

Patil BL, Fauquet C: Differential interaction between cassava mosaic geminiviruses and geminivirus satellites. J Gen Virol 2010. vir0019513-0v1-vir0019513-0

Bull SE, Briddon RW, Markham PG: Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNAβ complexes. Mol Biotechnol 2003, 23: 83-86. 10.1385/MB:23:1:83

Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG: Universal primers for the PCR-mediated amplification of DNAβ: a molecule associated with some monopartite begomoviruses. Mol Biotechnol 2002, 20: 315-318. 10.1385/MB:20:3:315

Zhou X, Liu Y, Robinson DJ, Harrison BD: Four DNA-A variants among Pakistani isolates of Cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from Okra. J Gen Virol 1998, 79: 915-923.

Tamura K, Dudley J, Nei M, Kumar S: MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24: 1596-1599. 10.1093/molbev/msm092

Thông tin
Thông tin xuất bản

Virology Journal

Tập 7 Số 1

Thông tin tác giả