Characterization of Isolates of <i>Streptococcus agalactiae</i> from Diseased Farmed and Wild Marine Fish from the U.S. Gulf Coast, Latin America, and Thailand

Journal of Aquatic Animal Health - Tập 27 Số 2 - Trang 123-134 - 2015
Esteban Soto1,2, Rui Wang3, Judy Wiles3, Wes Baumgartner4, Christopher Green5, John A. Plumb6,7, John P. Hawke3
1Department of Biomedical Sciences, School of Veterinary Medicine, Ross University, Post Office Box 334, Basseterre, St. Kitts, West Indies
2Present address: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, Louisiana, 70803 USA
3Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, Louisiana, 70803 USA
4Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, Mississippi State, Mississippi, 39762 USA
5Aquaculture Research Station, Louisiana State University Agricultural Center, 2410 Ben Hur Road, Baton Rouge, Louisiana, 70820 USA
6Retired
7School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, Auburn, Alabama, 36830 USA

Tóm tắt

AbstractWe examined Lancefield serogroup B Streptococcus isolates recovered from diseased, cultured hybrid Striped Bass (Striped Bass Morone saxatilis × White Bass M. chrysops) and wild and cultured Gulf Killifish Fundulus grandis from coastal waters of the U.S. Gulf of Mexico (Gulf coast) and compared those isolates to strains from tilapias Oreochromis spp. reared in Mississippi, Thailand, Ecuador, and Honduras and to the original Gulf coast strain identified by Plumb et al. (1974). The isolates were subjected to phylogenetic, biochemical, and antibiotic susceptibility analyses. Genetic analysis was performed using partial sequence comparison of (1) the 16S ribosomal RNA (rRNA) gene; (2) the sipA gene, which encodes a surface immunogenic protein; (3) the cspA gene, which encodes a cell surface‐associated protein; and (4) the secY gene, which encodes components of a general protein secretion pathway. Phylogenies inferred from sipA, secY, and cspA gene sequence comparisons were more discriminating than that inferred from the 16S rRNA gene sequence comparison. The U.S. Gulf coast strains showed a high degree of similarity to strains from South America and Central America and belonged to a unique group that can be distinguished from other group B streptococci. In agreement with the molecular findings, biochemical and antimicrobial resistance analyses demonstrated that the isolates recovered from the U.S. Gulf coast and Latin America were more similar to each other than to isolates from Thailand. Three laboratory challenge methods for inducing streptococcosis in Gulf Killifish were evaluated—intraperitoneal (IP) injection, immersion (IMM), and immersion plus abrasion (IMMA)—using serial dilutions of S. agalactiae isolate LADL 97‐151, a representative U.S. Gulf coast strain. The dose that was lethal to 50% of test fish by 14 d postchallenge was approximately 2 CFU/fish via IP injection. In contrast, the fish that were challenged via IMM or IMMA presented cumulative mortality less than 40% by 14 d postchallenge.Received July 31, 2014; accepted March 11, 2015

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Journal of Aquatic Animal Health

Tập 27 Số 2

123-134

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