Characterization of Bacterium Isolated from the Sediment at Coastal Area of Omura Bay in Japan and Several Biological Activities of Pigment Produced by This Isolate

Microbiology and Immunology - Tập 49 Số 5 - Trang 407-415 - 2005
Takuji Nakashima1,2, Maki Kurachi1, Munehiro Nakata2, Kenichi Yamaguchi1, Tatsuya Oda1
1Division of Biochemistry, Faculty of Fisheries, Nagasaki University, Nagasaki, Nagasaki, 852-8521 Japan
2Nagasaki Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST, Omura, Nagasaki, 856-0026 Japan

Tóm tắt

Abstract

Recently we discovered a bacterial strain (MS‐02–063) that produces large amounts of red pigment from coastal area of Nagasaki Prefecture, Japan. Comparative 16S rDNA gene sequencing analysis revealed that strain MS‐02–063 was phylogenetically closely related to γ‐proteobacterium Hahella sp. MBIC 3957 that produces prodigiosin. However, some physiological and biochemical differences between strain MS‐02–063 and Hahella sp. MBIC 3957 were observed. The red pigment (RP‐063) produced by this isolate was highly purified from the culture supernatant. It was speculated that RP‐063 might be prodigiosin‐like pigment in physical properties and biological activities such as antibacterial and cytotoxic activity. Antibacterial activity of RP‐063 was examined by an agar dilution method. The results indicated that RP‐063 showed antibacterial activity for specific for pathogenic gram‐positive bacteria such as Staphylococcus aureus. The potency of antibacterial activity against S. aureus was nearly equal to those of tetracycline. Moreover, RP‐063 showed inhibition of the superoxide generation by 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA)‐stimulated mouse macrophage RAW 264.7 cell line. Prodigiosin members have a wide variety of biological properties, including anticancer and antimalarial, etc. Especially, potent immunosuppressive properties have been reported for prodigiosin members with the mechanism of action different from that of the other well known immunosuppressors in atopic dermatitis therapy such as cyclosporin A, FK506 and rapamycin. It is suggested that RP‐063 may be able to arrest the inflammation caused by superantigens secreted from S. aureus, which colonized skin on atopic dermatitis as well as suppression of activated lymphocyte proliferation and superoxide generation from leucocytes.

Từ khóa


Tài liệu tham khảo

10.1038/216929a0

10.1016/0076-6879(86)33085-4

Anuradha G., 2004, A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil, BMC Microbiol., 18, 11

10.1067/mai.2000.105528

Fleischer B., 1991, An evolutionary conserved mechanism of T cell activation by microbial toxins. Evidence for different affinities of T cell receptor‐toxin interaction, J. Immunol., 146, 11, 10.4049/jimmunol.146.1.11

10.1074/jbc.272.30.18515

Giembycz M.A., 1999, Pharmacology of the eosinophil, Pharmacol. Rev., 51, 213

10.1139/m76-097

Gerber N.N., 1975, Prodiginine (prodigiosin‐like) pigments from Streptoverticillium rubrireticuli, an organism that causes pink staining of polyvinyl chloride, Appl. Microbiol., 30, 807, 10.1128/am.30.5.807-810.1975

10.1093/jb/mvg224

10.1093/infdis/165.6.1064

Han S.B., 2001, Prodigiosin blocks T cell activation by inhibiting interleukin‐2R alpha expression and delays progression of autoimmune diabetes and collagen‐induced arthritis, J. Pharmacol. Exp. Ther., 299, 415

10.1006/abio.1999.4107

10.1128/AAC.46.4.1112-1113.2002

10.1172/JCI110149

10.1084/jem.175.2.387

10.1073/pnas.87.6.2264

10.1016/0014-5793(94)01446-8

Lee K., 2001, Hahella chejuensis gen. nov., sp. nov., an extracellular‐polysaccharide‐producing marine bacterium, Int. J. Syst. Evol. Microbiol., 51, 661, 10.1099/00207713-51-2-661

10.1046/j.1365-2567.2000.00961.x

10.1172/JCI116711

10.1111/j.1365-2133.1993.tb00257.x

10.1021/tx025507x

10.1084/jem.177.2.283

10.1016/S0378-4274(01)00477-5

10.1159/000246218

Morrison D.A., 1966, Prodigiosin synthesis in mutants of Serratia marcescens, J. Bacteriol., 91, 1599, 10.1128/jb.91.4.1599-1604.1966

Mortellaro A., 1999, New immunosuppressive drug PNU156804 blocks IL‐2‐dependent proliferation and NF‐kappa B and AP‐1 activation, J. Immunol., 162, 7102, 10.4049/jimmunol.162.12.7102

National Committee for Clinical Laboratory Standards, 2000, Method for dilution antimicrobial susceptibility testing for bacteria that grow aerobically, 5th ed, Approved standard M7–A5

10.1139/w97-150

10.1016/S1567-5769(02)00114-5

Patel K.A., 1982, Prodigiosin, a component of kappa phage receptor complex in Serratia marcescens, Microbios, 34, 153

10.1038/187349a0

Saitou N., 1987, The neighbor‐joining method: a new method for reconstructing phylogenetic trees, Mol. Biol. Evol., 4, 406

10.1099/00207713-48-3-769

Smibert R.M., 1994, Methods for general and molecular bacteriology, 607

Songia S., 1997, Characterization of the new immunosuppressive drug undecylprodigiosin in human lymphocytes: retinoblastoma protein, cyclin‐dependent kinase‐2, and cyclin‐dependent kinase‐4 as molecular targets, J. Immunol., 158, 3987, 10.4049/jimmunol.158.8.3987

10.1016/j.bcp.2004.05.056

10.1093/nar/22.22.4673

10.1016/S0041-1345(00)02794-9

10.1002/1097-0215(20001001)88:1<121::AID-IJC19>3.0.CO;2-C

10.1139/o62-114

Thông tin
Thông tin xuất bản

Microbiology and Immunology

Tập 49 Số 5

407-415

Thông tin tác giả