Characterization by high performance liquid chromatography (HPLC) of the solubilization of phosphorus in iron ore by a fungus

Oxford University Press (OUP) - Tập 5 - Trang 183-189 - 1990
Edwin J. Parks1, Gregory J. Olson1, Frederick E. Brinckman1, Franco Baldi2
1Polymers Division, National Institute of Standards and Technology, Gaithersburg, U.S.A.
2Department of Environmental Biology, University of Siena, Italy

Tóm tắt

The value of iron ore is adversely affected by phosphorus in concentrations over 0.03% by weight. The present research concerns the use of metabolic products of aPenicillium-like fungus to leach insoluble phosphates (hydroxyapatite) from ores. Ion chromatography was used to measure metabolism of glucose into acidic fragments. The rate and products of glucose degradation depended on both the chemical composition of the growth medium (buffered or not) and incubation conditions (shaken or quiescent). The principal products were identified as oxalic acid and isomers of propylene dicarboxylic acid, mainly itaconic acid. Continued, slow metabolism of itaconic acid generates more oxalic acid. Aliphatic acids were not detected. Both iron ore phosphate and calcium phosphate were partially solubilized by either the spent broth or aqueous oxalic acid. Solubilization of ore phosphorus was greatly assisted by hydrochloric acid added to the spent broth in small increments. The data suggest biological alternatives to costly leaching procedures that use only mineral acids.

Tài liệu tham khảo

Waksman, S. 1932. Principles of Soil Microbiology, pp. 576–580, Williams and Wilkins, Baltimore.

Ehrlich, H.L. 1981. Geomicrobiology, pp. 140–141, Marcel Dekker, Inc., New York.

Tuovinen, O.H., P. Hiltunen and A. Vuorinen. 1983. Solubilization of phosphate, uranium, and iron from apatite-and uranium-containing rock samples in synthetic and microbiologically produced acid leach solutions. Eur. J. Appl. Microbiol. Biotechnol. 17: 327–333.

Banik, S. and B.K. Dey. 1981. I. Solubilization of inorganic phosphates and production of organic acids by microorganisms isolated in sucrose calcium phosphate agar plates. Zbl. Bakt. II Abt. 136: 478–486.

Babenko, Yu.S., G.I. Tyrgina, E.F. Grigor'ev, L.M. Dolgikh and T.I. Borisova. 1984. Biological activity and physiological-biochemical properties of phosphate-dissolving bacteria. Mikrobiologiya 53 (4): 533–539.

Banik, S. and B.D. Rey. 1983. Phosphate-solubilizing potentiality of microorganisms capable of utilizing aluminum phosphate as a sole phosphate source. Zbl. Microbiol. 138: 17–23.

Banik, S. and B.K. Dey. 1983. Alluvial soil microorganisms capable of utilizing insoluble aluminum phosphate as a sole source of phosphorus. Zbl. Mikrobiol. 138: 437–442.

Sperber, J.I. 1957. Solution of mineral phosphates by soil bacteria. Nature 180: 994–995.

Sperber, J.L. 1958. Solution of apatite by soil microorganisms producing organic acids. Australian. J. Agric. Research 9: 782–787.

Agnihotri, V.P. 1970. Solubilization of insoluble phosphates by some soil fungi isolated from nursery seedbeds. Can. J. Microbiol. 16: 877–880.

Duff, R.B., D.M. Webley and R.O. Scott. 1963. Solubilization of minerals and related materials by 2-ketogluconic acid producing bacteria. Soil Sci. 95: 105–114.

Duff, R.B. and D.M. Webley. 1959. 2-ketogluconic acid as a natural chelator produced by soil bacteria. Chem. Ind. 1376–1377.

Johnston, H.W. 1954. The solubilization of insoluble phosphate. A quantitative and comparative study of the action of selected aliphatic acids on tricalcium phosphate. N. Z. J. Sci. and Technol. 36: 49–55.

Sperber, J.I. 1958. Release of phosphate from soil minerals by hydrogen sulfide. Nature 181: 934.

Sethi, R.P. and N.S. Subba-Rao. 1968. Solubilization of tricalcium phosphate and calcium phytate by soil fungi. J. Gen. Appl. Microbiol. 14: 329–331.

Marshall, P. 1984. Austenitic Stainless Steels. Microstructure and Mechanical Properties, p. 254. Elsevier Applied Sciences, London, New York.

Sèdriks, A.J. 1979. Corrosion of Stainless Steels, p. 135, Wiley Interscience, New York.

Guerrant, G.O., M.A. Lambert and C.W. Moss. 1982. Analysis of short-chain acids from anaerobic bacteria by highperformance liquid chromatography. J. Clin. Microbiol. 16: 355–360.

Krausse, R. and U. Ullman. 1987. Identification of anaerobic bacteria using high-performance liquid chromatography. Zbl. Bakt. Hyg. A 265: 340–352.

Lockwood, L.B. 1954. In: Industrial Fermentations, Vol. I. (Underkofler, L.A. and R.J. Hickey, eds.), pp. 488, Chemical Publishing Co., New York.

Murphy, J. and J.P. Riley. 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta 27: 31–36.

American Society for Testing and Materials. 1985. Standard Test Method for Phosphorus in Iron Ores by the Phosphomolybdenum-Blue Photometric Methods. Method E 1070-85, pp. 930–932. In: Annual Book of ASTM Standards, vol. 3.05, ASTM, Philadelphia, PA.

Alexopoulos, C.J. 1962. Introductory Mycology, pp. 278–281, Wiley and Sons, Inc., New York.

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Oxford University Press (OUP)

Tập 5

183-189

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