Characterisation of the meningococcal transferrin binding protein complex by photon correlation spectroscopy
Tóm tắt
Photon correlation spectroscopy demonstrated for the first time that co‐purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species underwent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar apparent sizes but showed distinctive size profiles suggesting that TbpA forms a largely homogeneous population while TbpB may produce more variable particle sizes under these conditions.
Tài liệu tham khảo
10.1139/m89-063
10.1139/m90-026
10.1111/j.1365-2958.1993.tb01657.x
10.1128/jb.174.18.5788-5797.1992
10.1128/jb.176.11.3162-3170.1994
10.1128/iai.33.2.555-564.1981
10.1128/iai.63.3.884-890.1995
10.1128/jb.178.5.1437-1444.1996
A.P.F. Turner I. Karube G.S. Wislon 1987 Oxford Scientific Publications Oxford 686 689
1985 Plenum Press New York V.A. Bloomfield R. Pecora Dynamic Light Scattering: Applications of Photon Correlation Spectroscopy 369
10.1128/iai.61.2.751-759.1993
10.1016/0264-410X(95)00055-6
10.1128/iai.62.7.2984-2990.1994
10.1038/227680a0
10.1042/bj1890541
10.1128/iai.60.8.3253-3261.1992
10.1006/mpat.1994.1085
1996 R. Pajen G. Chirea E. Morrero G. Guillen Proc. 10th Int. Path. Neiss. Conf 580 581
10.1016/0959-440X(95)80034-4
Malvern Applications Handbook. (MAN 0065). Basics of Light Scattering. Malvern Instruments Ltd. Malvern pp. 2.11–2.12.
10.1002/bip.1976.360150706
J.M. Hollas 1996 Wiley Chichester 312 314