Changes in skin mucus of common carp, Cyprinus carpio L., after exposure to water with a high bacterial load

Journal of Fish Diseases - Tập 33 Số 5 - Trang 431-439 - 2010
M. van der Marel1, N Caspari2, H. Neuhaus2, Wieland Meyer3, M.‐L. Enss4, Dieter Steinhagen2
1Fish Disease Research Unit, Centre of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany
2Fish Disease Research Unit, Centre of Infectious Diseases, University of Veterinary Medicine, Hannover, Germany
3 Anatomical Institute, University of Veterinary Medicine Hannover, Germany
4Institute for Laboratory Animal Science and Animal Facility, Medical School of Hannover, Germany

Tóm tắt

AbstractWater in aquaculture systems may contain a high load of microorganisms. Reduction in overall bacterial tank water load improves fish health and growth parameters. In this study, the effect of an increase of overall bacterial load in tank water on carp skin mucus was assessed. Intracellular and released high molecular weight glycoproteins (HMGs) of carp skin mucus were analysed for changes using histological, histochemical and biochemical techniques. Increase of bacterial load did not induce obvious clinical responses in carp, but the skin of exposed carp responded quickly. The amount of skin mucus HMGs isolated increased as well as their total glycosylation. An increased goblet cell number was observed for all carbohydrate stainings, but most clearly for acidic glycoconjugates. A change in the terminal presence of some sugars was also seen. After the initial response of carp, an adaptation to the higher bacterial load in the water appeared to occur as mucins had a higher glycosylation. The changes observed suggest that these skin mucus adaptations are part of a primary defence mechanism of mucosal epithelia, even at a low pathogenic pressure.

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Journal of Fish Diseases

Tập 33 Số 5

431-439

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