Changes in T, B, and NK Lymphocyte Subsets During and After Normal Pregnancy

American Journal of Reproductive Immunology - Tập 37 Số 5 - Trang 368-377 - 1997
Mikio Watanabe1, Yoshinori Iwatani2, T Kaneda3, Yoh Hidaka3, Nobuaki Mitsuda4, Yasuhiko Morimoto5, Nobuyuki Amino3
1Department of Laboratory Medicine, Osaka University Medical School, Japan
2Department of Clinical Laboratory Science, School of Allied Health Sciences, Faculty of Medicine, Osaka University, Suita, Osaka, Japan
3Department of Laboratory Medicine, Osaka University Medical School, Suita, Osaka, Japan
4Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
5Aizenbashi Hospital, Osaka, Japan

Tóm tắt

PROBLEM: Pregnancy affects the maternal immune system and the clinical course of maternal diseases. Here we report the changes in the detailed lymphocyte subsets of helper T cells, suppressor T cells, CD5+ B cells, T cell receptor (TCR) αβ‐positive T cells (Tαβ cells), TCRαβ‐negative T cell (Tγδ cells), and others during and after pregnancy through to one year postpartum, and discuss the significance of the changes.

METHOD: The absolute numbers of helper T cells, suppressor T cells, cytotoxic T cells, TCRαβ‐negative T cells (Tγδ cells), CD5 B cells, CD5+ B cells, and NK cell subsets were examined by two‐color flow cytometry in peripheral blood from 51 healthy non‐pregnant women, 106 healthy pregnant women, and 148 healthy postpartum women.

RESULTS: In early pregnancy, the numbers of suppressor T cells and NK cells with strong cytotoxicity (NK+++ cells) increased, and the number of cytotoxic T cells decreased. In late pregnancy, the helper T cell and NK+++ cell numbers decreased. Tαβ, CD5 B and CD5+ B cells decreased during pregnancy. After delivery, helper T cells and cytotoxic T cells increased from 1 to 4 months postpartum, and suppressor T cells increased at 7 months postpartum. TCRαβ‐negative T cells increased at 4 to 10 months postpartum. Both CD5 and CD5+ B cells decreased further at 1 month postpartum, but CD5+ B cells increased markedly at 7 to 10 months postpartum.

CONCLUSIONS: These data indicate that 1) early increases of suppressor T cells and NK+++ cells during pregnancy may be related to the mechanism to accept or reject the fetus in early pregnancy, respectively; 2) late decreases of helper T cells and NK+++ cells may be related to the maintenance of pregnancy: 3) postpartum increases of helper T cells, cytotoxic T cells, TCRαβ‐negative T cells (Tγδ cells), and CD5+ B cells may be related to the postpartum aggravation of autoimmune diseases; and 4) the immunological effects of pregnancy remains until about 1 year after delivery.

Từ khóa


Tài liệu tham khảo

Gleicher N., 1983, Autoimmune endocrine disease, 225

10.1111/j.1600-0897.1996.tb00489.x

10.1615/CritRevImmunol.v14.i3-4.30

10.1111/j.1600-0897.1988.tb00235.x

Plum J., 1978, Distribution of mononuclear cells during pregnancy, Clin Exp Immunol, 31, 45

Rubin LA, 1985, Soluble interleukin 2 receptors are released from activated human lymphoid cells in vitro, J Immunol, 135, 3172, 10.4049/jimmunol.135.5.3172

10.1016/0165-0378(89)90025-9

10.1016/0090-1229(91)90109-N

10.1016/0165-0378(89)90042-9

10.1016/0165-0378(91)90024-K

Amino N., 1978, Changes of serum immunoglobulins IgG, IgA, IgM, and IgE during pregnancy, Obstet Gynecol, 52, 415

Amino N., 1978, Changes of serum anti‐thyroid antibodies during and after pregnancy in autoimmune thyroid disease, Clin Exp Immunol, 31, 30

10.1111/j.1600-0897.1982.tb00169.x

Amino N., 1983, Autoimmune endocrine disease, 247

10.1056/NEJM198204083061405

10.1056/NEJM196207262670401

10.1136/ard.12.3.227

10.1111/j.1600-0897.1996.tb00010.x

Stites DP., 1994, Basic and clinical immunology, 195

10.1111/j.1365-2249.1992.tb03017.x

10.1007/BF00917143

Gatenby PA, 1982, Dissection of immunoregulatory subpopulations of T lymphocytes within the helper and suppressor sublineages in man, J Immunol, 129, 1997, 10.4049/jimmunol.129.5.1997

Landay A., 1983, Characterization of a phenotypically distinct subpopulation of Leu‐2+ cells that suppresses T cell proliferative responses, J Immunol, 131, 2757, 10.4049/jimmunol.131.6.2757

10.1016/0090-1229(89)90066-4

10.4049/jimmunol.131.4.1789

10.1002/eji.1830140707

10.1111/j.1600-0897.1996.tb00493.x

10.1111/j.1600-0897.1995.tb00888.x

10.1111/j.1600-0897.1996.tb00167.x

10.1016/0165-0378(90)90012-U

Imboden JB., 1994, Basic and clinical immunology, 94

Gilbertson SM, 1986, NK cells suppress the generation of LYT‐2+ cytolytic T cells by suppressing or eliminating dendritic cells, J Immunol, 136, 3567, 10.4049/jimmunol.136.10.3567

Brieva JA, 1984, NK and T cell subsets regulate antibody production by human in vivo antigen‐induced lymphoblastoid B cells, J Immunol, 132, 611, 10.4049/jimmunol.132.2.611

10.1016/0165-0378(93)90002-Y

10.1146/annurev.immunol.14.1.397

10.1016/S0950-351X(95)80819-1

10.1016/0165-0378(94)00907-O

10.1146/annurev.iy.06.040188.001213

Iwatani Y., 1989, Marked increase of CD5+ B cells in hyperthyroid Graves' disease, Clin Exp Immunol, 78, 196

10.1016/0952-7915(93)90105-2

Volpé R., 1991, The Thyroid Philadelphia, 648

10.1111/j.1600-0897.1995.tb00924.x

10.1016/0167-5699(94)90212-7

10.1084/jem.178.5.1507

Athanassakis I., 1987, The immunostimulatory effect of T cells and T cell lymphokines on murine fetally derived placental cells, J Immunol, 138, 37, 10.4049/jimmunol.138.1.37

Clark DA, 1988, Active suppression of host vs. graft reaction in pregnant mice, J Immunol, 141, 3833

Chaouat G., 1995, IL‐10 prevents naturally occurring fetal loss in the CBA x DBA/2 mating combination, and local defect in IL‐10 production in this abortion‐prone combination is corrected by in vivo injection of IFN‐τ, J Immunol, 154, 4261, 10.4049/jimmunol.154.9.4261

10.1111/j.1600-0897.1996.tb00490.x

10.1111/j.1365-2249.1993.tb08196.x

Scott JR, 1990, Autoimmune disease in pregnancy, Immunol Allergy Clin North AM, 10, 118, 10.1016/S0889-8561(22)00253-3

10.1007/978-1-4684-5973-9_8