Cellular immune response profile in patients with American tegumentary leishmaniasis prior and post chemotherapy treatment

Journal of Clinical Laboratory Analysis - Tập 23 Số 1 - Trang 63-69 - 2009
Luiza Campos Reis1, Maria E. F. de Brito1, Marina de Assis Souza1, Ângela Cristina Rapela Medeiros2, Claudio J. Silva3, Carlos Feitosa Luna1, Valéria Rêgo Alves Pereira4
1Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães (CPqAM/FIOCRUZ), Recife, PE, Brazil
2Hospital Oswaldo Cruz—HUOC, Universidade de Pernambuco/UPE, Recife, PE, Brazil
3Núcleo de Vigilância em Saúde e Ambiente do Município de Moreno, Moreno, PE, Brazil
4Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães—CPqAM/Fiocruz, Recife, PE, Brazil

Tóm tắt

Abstract

In this study, we have the objective of evaluating the lymphoproliferative response and determining interferon (IFN)‐γ and interleukin (IL)‐10 cytokine production in the peripheral blood mononuclear cells (PBMC) of patients with American tegumentary leishmaniasis prior and post 12 months of chemotherapy treatment with meglumine antimoniate compared with the PBMC of noninfected donors. Lymphoproliferation, such as cytokine production, was evaluated through in vitro stimulus with the soluble antigenic fraction from Leishmania (Viannia) braziliensis promastigotes (1.25 µg/ml) and Concanavalin A (2.5 µg/ml). Patients showed a significant lymphoproliferative response prior and post treatment compared with the control group. Similar result, prior to chemotherapy treatment, was observed in IFN‐γ and IL‐10 production when patients were compared with the control group. After chemotherapy treatment, PBMC lymphoproliferative response of the patients revealed an increase, whereas patients have shown a decrease in IFN‐γ levels and an increase in IL‐10, although without statistical difference. These results may indicate that the patients produced a specific cellular response to the soluble antigenic fraction suggesting that besides Th1 and Th2 dichotomy, immunological regulation mechanisms with the participation of memory T cells and regulatory T cells could be present in the clinical evolution of these patients. This understanding will allow the study and identification of new L. (V.) braziliensis molecules potentially candidates to vaccines. J. Clin. Lab. Anal. 23:63–69, 2009. © 2009 Wiley‐Liss, Inc.

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Journal of Clinical Laboratory Analysis

Tập 23 Số 1

63-69

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