Cellular and humoral immune responses of Senegalese sole, Solea senegalensis (Kaup), following challenge with two Photobacterium damselae subsp. piscicida strains from different geographical origins

Journal of Fish Diseases - Tập 36 Số 6 - Trang 543-553 - 2013
Benjamín Costas1,2,3, P.C.N.P. Rêgo2,3, I Simões2,3, Joana Marques2,4, M. Castro‐Cunha5, António Afonso2,3
1CCMAR–CIMAR L.A. Centro de Ciências do Mar do Algarve Faro Portugal
2CIIMAR–CIMAR L.A. Centro Interdisciplinar de Investigação Marinha e Ambiental Porto Portugal
3ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal
4Edifício FC Faculdade de Ciências Universidade do Porto Porto Portugal
5A. Coelho & Castro Lda. Praça Luís de Camões Póvoa de Varzim Portugal

Tóm tắt

AbstractThe present study aimed to investigate leucocyte responses to inflammation as well as some innate immune parameters of Senegalese sole, Solea senegalensis, following challenge with two strains of Photobacterium damselae subsp. piscicida belonging to the European and Japanese clones described for this bacterium. Pathogenicity assays were performed to assess the virulence of each Photobacterium damselae subsp. piscicida strain for sole. Subsequently, fish were intraperitoneally injected with phosphate‐buffered saline (control) or two concentrations (2 × 102 and 2 × 106 CFU mL−1) of each bacterial strain and sampled after 6 and 24 h. Results showed that the European isolate induces a higher degree of response than the Japanese strain. While blood neutrophilia and monocytosis correlated well with the increase in neutrophil and macrophage numbers in the peritoneal cavity, fish infected with the European isolate presented higher peritoneal cell numbers than fish challenged with the Japanese strain. In addition, alternative complement pathway activity and respiratory burst of head kidney leucocytes increased significantly in fish infected with the European isolate. The enhanced innate immune response displayed by Senegalese sole challenged with the European isolate is probably due to the higher degree of virulence presented by this Photobacterium damselae subsp. piscicida strain.

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Thông tin xuất bản

Journal of Fish Diseases

Tập 36 Số 6

543-553

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