Cell wall structure suitable for surface display of proteins in <i>Saccharomyces cerevisiae</i>

Yeast - Tập 31 Số 2 - Trang 67-76 - 2014
Hiroyuki Matsuoka1, Kazuya Hashimoto1, Aki Saijo1, Yuki Takada1, Akihiko Kondo2, Mitsuyoshi Ueda3, Hiroshi Ooshima1, Taro Tachibana1, Masayuki Azuma1
1Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University, Japan
2Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Japan
3Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan

Tóm tắt

AbstractA display system for adding new protein functions to the cell surfaces of microorganisms has been developed, and applications of the system to various fields have been proposed. With the aim of constructing a cell surface environment suitable for protein display in Saccharomyces cerevisiae, the cell surface structures of cell wall mutants were investigated. Four cell wall mutant strains were selected by analyses using a GFP display system via a GPI anchor. β‐Glucosidase and endoglucanase II were displayed on the cell surface in the four mutants, and their activities were evaluated. mnn2 deletion strain exhibited the highest activity for both the enzymes. In particular, endoglucanase II activity using carboxymethylcellulose as a substrate in the mutant strain was 1.9‐fold higher than that of the wild‐type strain. In addition, the activity of endoglucanase II released from the mnn2 deletion strain by Zymolyase 20T treatment was higher than that from the wild‐type strain. The results of green fluorescent protein (GFP) and endoglucanase displays suggest that the amounts of enzyme displayed on the cell surface were increased by the mnn2 deletion. The enzyme activity of the mnn2 deletion strain was compared with that of the wild‐type strain. The relative value (mnn2 deletion mutant/wild‐type strain) of endoglucanase II activity using carboxymethylcellulose as a substrate was higher than that of β‐glucosidase activity using p‐nitrophenyl‐β‐glucopyranoside as a substrate, suggesting that the cell surface environment of the mnn2 deletion strain facilitates the binding of high‐molecular‐weight substrates to the active sites of the displayed enzymes. Copyright © 2014 John Wiley & Sons, Ltd.

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Yeast

Tập 31 Số 2

67-76

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