Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification

International Endodontic Journal - Tập 53 Số 4 - Trang 467-477 - 2020
Y. Liu1,2, Xinming Liu1,2, Jing Bi1,2, Si Yu1,2, Ning Yang1,2, Bing Song3, Xu Chen1,2
1Department of Paediatric Dentistry, School of Stomatology, China Medical University, Shenyang, China
2Liaoning Province Key Laboratory of Oral Disease, Shenyang, China
3School of Dentistry, Cardiff University, Cardiff, UK

Tóm tắt

AbstractAimTo investigate the in vitro biological effects of a nanoparticle bioceramic material, iRoot Fast Set root repair material (iRoot FS), on the proliferation, migration and osteo/odontogenic differentiation of human stem cells from the apical papilla (hSCAP), and to further explore the mechanism involved in osteo/odontogenic induction of iRoot FS.MethodologyhSCAP were isolated and characterized in vitro. iRoot FS conditioned medium were prepared and used to treat hSCAP, while using mineral trioxide aggregate (MTA) conditioned medium as the positive control and regular medium as the negative control. MTT assay and BrdU labelling assay were performed to determine cell proliferation. Wound healing assay and transwell assay were conducted to evaluate cell migration. The osteo/odontogenic differentiation of hSCAP was evaluated by qPCR, Western blot and Alizarin red S staining. Wnt inhibitor was used for downregulating the expression level of β‐catenin of hSCAP.ResultsThe cell proliferation of hSACP in the iRoot FS group was not significantly different compared with the control groups. The cell migration of hSCAP in the iRoot FS group was significantly increased than the MTA and negative control groups (P < 0.01). The expression levels of osteo/odontogenic markers and mineralization nodule formation of hSCAP in the iRoot FS group were significantly elevated (P < 0.01). Furthermore, iRoot FS enhanced the osteo/odontogenic differentiation of hSCAP by activating Wnt/β‐catenin signalling.ConclusionsiRoot FS promoted the cell migration of hSCAP and enhanced their oseto/odontogenesis potential via the Wnt/β‐catenin pathway without cytotoxicity. iRoot FS had satisfactory biological properties and has potential to be used as an apical barrier in apexification or as a coronal sealing material in regenerative endodontic treatment.

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International Endodontic Journal

Tập 53 Số 4

467-477

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