Cell Death in Pseudomonas aeruginosa Biofilm Development

Journal of Bacteriology - Tập 185 Số 15 - Trang 4585-4592 - 2003
Jeremy S. Webb1, Lyndal S. Thompson1, S. R. James1, Tim Charlton1, Tim Tolker‐Nielsen2, Birgit Koch2, Michael Givskov2, Staffan Kjelleberg1
1School of Biotechnology and Biomolecular Sciences, and Centre for Marine Biofouling and Bio-innovation, University of New South Wales, Sydney, NSW 2052, Australia
2BioCentrum, Technical University of Denmark, DK-2800 Lyngby, Denmark

Tóm tắt

ABSTRACT Bacteria growing in biofilms often develop multicellular, three-dimensional structures known as microcolonies. Complex differentiation within biofilms of Pseudomonas aeruginosa occurs, leading to the creation of voids inside microcolonies and to the dispersal of cells from within these voids. However, key developmental processes regulating these events are poorly understood. A normal component of multicellular development is cell death. Here we report that a repeatable pattern of cell death and lysis occurs in biofilms of P. aeruginosa during the normal course of development. Cell death occurred with temporal and spatial organization within biofilms, inside microcolonies, when the biofilms were allowed to develop in continuous-culture flow cells. A subpopulation of viable cells was always observed in these regions. During the onset of biofilm killing and during biofilm development thereafter, a bacteriophage capable of superinfecting and lysing the P. aeruginosa parent strain was detected in the fluid effluent from the biofilm. The bacteriophage implicated in biofilm killing was closely related to the filamentous phage Pf1 and existed as a prophage within the genome of P. aeruginosa . We propose that prophage-mediated cell death is an important mechanism of differentiation inside microcolonies that facilitates dispersal of a subpopulation of surviving cells.

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Journal of Bacteriology

Tập 185 Số 15

4585-4592

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