Carbodiimide Inactivation of MMPs and Effect on Dentin Bonding

SAGE Publications - Tập 93 Số 3 - Trang 263-268 - 2014
Annalisa Mazzoni1, Fabianni Magalhães Apolônio2, V P A Sabóia2, Spartaco Santi3,4, V. Angeloni1, Vittorio Checchi1, Rosa Curci4, Roberto Di Lenarda1, Franklin R. Tay5,6, D.H. Pashley5, Lorenzo Breschi7,3
1Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Italy
2Department of Dentistry, Universidade Federal Do Ceara, Fortaleza, Brazil
3IGM-CNR, Unit of Bologna, Italy
4Laboratory of Musculoskeletal Cell Biology, RAMSES, IOR, Bologna, Italy
5Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USA
6Endodontics
7Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna and IGM-CNR, Unit of Bologna, Italy

Tóm tắt

The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy showed that hybrid layers of tested adhesives exhibited intense collagenolytic activity, while almost no fluorescence signal was detected when specimens were pre-treated with EDC. The correlative analysis used in this study demonstrated that EDC could contribute to inactivate endogenous dentin MMPs within the hybrid layer created by etch-and-rinse adhesives.

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SAGE Publications

Tập 93 Số 3

263-268

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