Calcium requirement and increased association with bovine sperm during capacitation by heparin

Wiley - Tập 252 Số 2 - Trang 174-182 - 1989
R. R. Handrow1, N. L. First2, J.J. Parrish3
1Department of Meat and Animal Science, University of Wisconsin-Madison 53706.
2Department of Meat and Animal Science, University of Wisconsin, Madison, Wisconsin 53706
3University of Wisconsin, 1675 Observatory Drive, Madison, WI 53706

Tóm tắt

AbstractThe requirement for external Ca+2 during capacitation of ejaculated bovine sperm with heparin and changes in sperm‐associated 45Ca+2 during capacitation were investigated in vitro. Sperm capacitation was evaluated by ability to undergo an acrosome reaction (AR) upon exposure to lysophosphatidylcholine. The percentage of sperm which were capacitated during a 4 h incubation with heparin increased exponentially with increased exposure time to 2 mM Ca+2. When sperm were incubated with or without heparin in the presence of 45CaCl2, there was no difference in the amount of 45Ca+2 associated with sperm initially or at 1 h of incubation. Incubation with heparin resulted in a greater amount of sperm‐associated 45Ca+2 at 2, 3, and 4 h as compared to sperm incubated without heparin. The amount of 45Ca+2 associated with sperm during capacitation was unaffected by washing with 2 mM EGTA‐5 mM LaCl3. Glucose (5 mM) inhibited the effects of heparin on sperm‐associated 45Ca+2 and on capacitation. The inhibitory effects of glucose could be overridden by 8‐bromo‐cAMP. The results suggest that the requirement for external Ca+2 during capacitation with heparin may be related to an increased association of external Ca+2 with sperm.

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Wiley

Tập 252 Số 2

174-182

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