Calcium currents of differentiated mouse neuroblastoma cells
Tóm tắt
Differentiated mouse neuroblastoma cells (line C 1300, clone N-18-TG2A1) were investigated by intracellular dialysis. A slow component was found in the potential-dependent inward current of these cells. The results of investigation of changes in amplitude of this component during variation of the ionic composition of the external and internal solutions showed that this component is due to transport of calcium ions. A calcium current was observed in all cells tested. The region of its activation was between −70 and −65 mV; maximal values of this current were reached when the membrane potential was between −30 and −40 mV. The kinetic characteristics of this current were examined. In its kinetics and potential-dependence, this calcium current of the mouse neuroblastoma cell membrane is analogous to the fast component of the calcium current in normal neurons of rat spinal ganglia.
Tài liệu tham khảo
N. S. Veselovskii and S. A. Fedulova, "Two types of calcium channels in the somatic membrane of rat spinal ganglia," Dokl. Akad. Nauk SSSR,268, No. 3, 747 (1983).
S. L. Mironov, "Determination of the power index of the activation variable of ionic channels for the Hodgkin-Huxley model," Neirofiziologiya,14, No. 2, 207 (1982).
N. Kh. Pogorelaya, A. F. Fomina, and N. S. Veselovskii, "Morphological differentiation of cells of a neuroblastoma induced by dimethylsulfoxide," Neirofiziologiya,16, No. 4, 519 (1984).
L. Byerly and S. Hagiwara, "Calcium currents in internally perfused cell bodies ofLimnea stagnalis," J. Physiol. (London),322, No. 2, 503 (1982).
M. C. Fishman and I. Spector, "Potassium current suppression by quinidine reveals additional calcium currents in neuroblastoma cells," Proc. Natl. Acad. Sci. USA,78, No. 8, 5245 (1981).
P. G. Kostyuk, O. A. Krishtal, and V. I. Pidoplichko, "Intracellular dialysis of nerve cells: effect of intracellular fluoride and phosphate on the inward currents," Nature,257, 691 (1975).
P. G. Kostyuk and O. A. Krishtal, "Separation of sodium and calcium currents in the somatic membrane of mollusc neurons," J. Physiol. (London),270, No. 3, 545 (1977).
P. G. Kostyuk, O. A. Krishtal, V. I. Pidoplichko, and N. S. Veselovskii (N. S. Veselovsky), "Ionic currents in the neuroblastoma cell membrane," Neuroscience,3, No. 3, 327 (1978).
P. G. Kostyuk, N. S. Veselovskii (N. S. Veselovsky), and A. Y. Tsyndrenko, "Ionic currents in the somatic membrane of rat dorsal root ganglion neurons. 1. Sodium currents," Neuroscience,6, No. 12, 2423 (1981).
P. G. Kostyuk, N. S. Veselovskii, and S. A. Fedulova, "Ionic currents in the somatic membrane of rat dorsal root ganglion neurons. 2. Calcium currents," Neuroscience,6, 2431 (1981).
W. H. Moolenaar and I. Spector, "Ionic currents in cultured mouse neuroblastoma cells under voltage clamp conditions," J. Physiol. (London),278, No. 1, 265 (1978).
W. H. Moolenaar and I. Spector, "The calcium current and the activation of a slow potassium conductance in voltage-clamped mouse neuroblastoma cells," J. Physiol. (London),292, No. 2, 307 (1979).
Yu. A. Shakhovalov, "Quantitative description of the Na and Ca inward currents in molluscan neurones by Hodgkin-Huxley equations," J. Physiol. (London),270, No. 3, 562 (1977).