CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

American Association for the Advancement of Science (AAAS) - Tập 315 Số 5819 - Trang 1709-1712 - 2007
Rodolphe Barrangou1,2,3, Christophe Fremaux1,2,3, Hélène Deveau1,2,3, Melissa Richards1,2,3, Patrick Boyaval1,2,3, Sylvain Moineau1,2,3, Dennis Romero1,2,3, Philippe Horvath1,2,3
1Danisco France SAS, Boîte Postale 10, F-86220 Dangé-Saint-Romain, France.
2Danisco USA, Inc., 3329 Agriculture Drive, Madison, WI 53716, USA.
3Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Félix d'Hérelle Reference Center for Bacterial Viruses, Université Laval, G1K 7P4 Québec, Canada

Tóm tắt

Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, together with associated cas genes, provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.

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We thank L. Bayer C. Vos and A.-C. Coûté-Monvoisin of Danisco Innovation as well as J. Labonté and D. Tremblay of Université Laval for technical support and E. Bech Hansen for discussions and critical review of the manuscript. Also we thank T. R. Klaenhammer for providing the integration system. This work was supported by funding from Danisco A/S. Also S. M. would like to acknowledge support from the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Program. Sequences were deposited in GenBank accession numbers EF434458 to EF434504.

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American Association for the Advancement of Science (AAAS)

Tập 315 Số 5819

1709-1712

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