CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

American Association for the Advancement of Science (AAAS) - Tập 360 Số 6387 - Trang 436-439 - 2018
Janice S. Chen1, Enbo Ma1, Lucas B. Harrington1, Maria Da Costa2, Xinran Tian3, Joel M. Palefsky2, Jennifer A. Doudna3,1,4,5,6
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA
3Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720 USA
4Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720 USA
5Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94704, USA.
6Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA

Tóm tắt

Taking CRISPR technology further CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al. added a sample preparation protocol to create a field-deployable viral diagnostic platform for rapid detection of specific strains of pathogens in clinical samples. Cas12a (also known as Cpf1), a type V CRISPR protein, cleaves double-stranded DNA and has been adapted for genome editing. Chen et al. discovered that Cas12a also processes single-stranded DNA threading activity. A technology platform based on this activity detected human papillomavirus in patient samples with high sensitivity. Science , this issue p. 439 , p. 444 , p. 436 ; see also p. 381

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American Association for the Advancement of Science (AAAS)

Tập 360 Số 6387

436-439

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