Takuo Onizuka1, Hideo Akiyama1, Sumiyo Endo1, Shozo Kanai1, Masahiko Hirano1, Satoshi Tanaka2, Hitoshi Miyasaka2
1Biological Science Laboratories, Toray Research Center, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan
2Technical Research Center, Kansai Electric Power Co., Inc., 3-11-20 Nakoji, Amagasaki, Hyogo 661-0974, Japan
Tóm tắt
The CO2‐regulatory function of the AT‐rich element in the promoter for ribulose‐1,5‐bisphosphate carboxylase/oxygenase (rbc) genes in the cyanobacterium Synechococcus sp. PCC7002 was analyzed using the transcription factor decoy approach. Double‐stranded phosphorothioate AT‐rich oligonucleotides with high affinity for a sequence‐specific DNA‐binding protein were successfully introduced into cyanobacterial cells in culture without any transfection reagent. The AT‐rich decoy oligonucleotides interfered with CO2 regulation of rbc expression by blocking the binding of the sequence‐specific DNA‐binding protein, indicating that the AT‐rich element plays a critical role in CO2 regulation for rbc genes. The decoy oligonucleotide approach to cyanobacteria provides a simple and excellent tool for investigating transcriptional regulation in vivo.
