CD14++CD16− and CD14+CD16+ human monocyte adhesion to endothelial cells

Cell and Tissue Biology - Tập 4 - Trang 322-325 - 2010
E. A. Starikova1, A. M. Lebedeva1, I. S. Freidlin1
1Institute of Experimental Medicine of Russian Academy of Medical Sciences, St. Petersburg, Russia

Tóm tắt

Based on the difference in the CD14 and CD16 expression, two subsets of monocytes were identified in human and other mammalian blood. These subsets have different patterns of adhesion molecules and chemokine receptors that suggests the different mode of their interaction with endothelium and tissue traffic. Here, we investigated the ability of CD14+CD16+ and CD14++CD16− monocytes to adhere to endothelial cell monolayer in presence or absence of pro- and anti-inflammatory cytokines. We demonstrated that CD14+CD16+ monocytes had a higher level of adhesion to intact monolayer of endothelial cells than CD14++CD16− monocytes. Adhesion of CD14++CD16− and CD14+CD16+ monocytes significantly increased in the presence of TNFα or its combination with other cytokines. IFNγ and IL-4 alone did not affect the adhesion of monocytes. These results show that CD14++CD16− and CD14+CD16+ monocytes can be recruited to the inflamed endothelium, but CD14+CD16+ monocytes adhere to endothelial cells without inflammations twice as strongly as CD14++CD16− monocytes.

Tài liệu tham khảo

Allen, J.B., Wong, H.L., Guyre, P.M., Simon, G.L., and Wahl, S.M., Association of Circulating Receptor Fcγ Riii-Positive Monocytes in Aids Patients with Elevated Levels of Transforming Growth Factor-β, J. Clin. Invest., 1991, vol. 87, pp. 1773–1779.

Ancuta, P., Rao Ravi, Moses, A., Mehle, A., Shaw, S.K., Luscinskas, F.W., and Gabuzda, D., Fractalkine Preferentially Mediates Arrest and Migration of Cd16+ Monocytes, J. Exp. Med., 2003, vol. 12, pp. 1701–1707.

Borgmann, S., Bayer, A., Knig, W., Ambrosch, A., and Kraus, J., Contrasting Effects of Long-Term Treatment with IFNγ in Endothelial Cells: Increase in Il-6 Secretion versus Decrease in Il-8 Secretion, NF-κB, and Ap-1 Activation, Endothelium, 2002, vol. 3, pp. 173–178.

Carlos, T.M., Leukocyte-Endothelial Adhesion Molecules, Blood, 1994, vol. 84, pp. 2068–2101.

Chen, C. and Manning, A., Tgf-Beta 1, Il-10 and Il-4 Differentially Modulate the Cytokine-Induced Expression of Il-6 and Il-8 in Human Endothelial Cells, Cytokine, 1996, vol. 8, pp. 58–65.

Colotta, F., Sironi, M., Borre, A., Luini, W., Maddalena, F., and Mantovani, A., Interleukin 4 Amplifies Monocyte Chemotactic Protein and Interleukin 6 Production by Endothelial Cells, Cytokine, 1992, vol. 4, pp. 24–28.

Fernandez, G.C., Ramos, M.V., Gomez, S.A., Dran, G.I., Exeni, R., Alduncin, M., Grimoldi, I., Vallejo, G., Elias-Costa, C., Isturiz, M.A., and Palermo, M.S., Differential Expression of Function-Related Antigens on Blood Monocytes in Children with Hemolytic Uremic Syndrome, J. Leukoc. Biol., 2005, vol. 7, pp. 853–861.

Fingerle, G., Pforte, A., Passlick, B., Blumenstein, M., Strobel, M., and Ziegler-Heitbrock, H.W., The Novel Subset of Cd14+/Cd16+ Blood Monocytes Is Expanded in Sepsis Patients, Blood, 1993, vol. 82, pp. 3170–3176.

Geissmann, F., Jung, S., and Littman, D.R., Blood Monocytes Consist of Two Principal Subsets with Distinct Migratory Properties, Immunity, 2003, vol. 19, pp. 71–82.

Glanc, S., Mediko-biologicheskaya statistika (Medical-Biological Statistic), Moscow: Praktika, 1999.

Goebeler, M., Yoshimura, T., Toksoy, A., Ritter, U., Brocker, E., and Gillitzer, R., The Chemokine Repertoire of Human Dermal Microvascular Endothelial Cells and Its Regulation by Inflammatory Cytokines, J. Invest. Dermatol., 1997, vol. 108, pp. 445–451.

Grage-Griebenow, E., Flad, H.D., Ernst, M., Bzowska, M., Skrzeczynska, J., and Pryjma, J., Human MO Subsets as Defined by Expression of Cd64 and Cd16 Differ in Phagocytic Activity and Generation of Oxygen Intermediates, Immunobiology, 2000, vol. 202, pp. 42–50.

Imhof, B.A. and Aurrand-Lions, M., Adhesion Mechanisms Regulating the Migration of Monocytes, Nature Reviews, 2004, vol. 4, pp. 432–444.

Kim, M., Carma, C.V., and Springer, T.A., Bidirectional Transmembrane Signaling by Cytoplasmic Domain Separation in Integrins, Science, 2003, vol. 301, pp. 1720–1725.

Mantovani, A., Bussolino, F., and Intora, M., Cytokine Regulation Endothelial Cell Function: From Molecular Level to Bedside, Immunology Today, 1997, vol. 18, pp. 231–239.

Mantovani, A., The Chemokine System: Redundancy for Robust Outputs, Immunology Today, 1999, vol. 20, pp. 254–257.

Marfaing-Koka, A., Devergne, O., Gorgone, G., Portier, A., Schall, T., Galanaud, P., and Emilie, D., Regulation of the Production of the Rantes Chemokine by Endothelial Cells. Synergistic Induction by Ifn-Gamma Plus Tnf-Alpha and Inhibition by Il-4 and Il-13, J. Immunol., 1995, vol. 154, pp. 1870–1878.

Meager, A., Cytokine Regulation of Cellular Adhesion Molecule Expression in Inflammation, Cytokine and Growth Factor Reviews, 1999, vol. 10, pp. 27–39.

Passlick, B., Flieger, D., and Loms Ziegler-Heitbrock, H.W., Identification and Characterization of a Novel Monocyte Subpopulation in Human Peripheral Blood, Blood, 1989, vol. 74, pp. 2527–2534.

Strauss-Ayali, D., Conrad, S.M., and Mosser, D.M.J., Monocyte Subpopulations and Their Differentiation Patterns during Infection, Leukoc. Biol., 2007, vol. 82, pp. 244–252.

Thieblemont, N., Weiss, L., Sadeghi, H.M., Estcourt, C., and Haeffner-Cavaillon, N., Cd14lowcd16high: A Cytokine-Producing Monocyte Subset Which Expands during Human Immunodeficiency Virus Infection, Eur. J. Immunol., 1995, vol. 25, pp. 3418–3424.

Von Andrian, U.H., and Mempel, T.R., Homing and Cellular Traffic in Lymph Nodes, Nature Reviews, 2003, vol. 3, pp. 867–878.

Weber, C., Belge, K.U., Von Hundelshausen, P., Draude, G., Steppich, B., Mack, M., Frankenberger, M., Weber, K.S.C., and Ziegler-Heitbrock, H.W.L., Differential Chemokine Receptor Expression and Function in Human Monocyte Subpopulations, J. Leukoc. Biol., 2000, vol. 67, pp. 699–704.

Ziegler-Heitbrock, H.W.L., The Cd14+Cd16+ Blood Monocytes: Their Role in Infection and Inflammation, J. Leukoc. Biol., 2007, vol. 81, pp. 584–592.

Thông tin
Thông tin xuất bản

Cell and Tissue Biology

Tập 4

322-325

Thông tin tác giả