CD134 and CXCR4 expression corresponds to feline immunodeficiency virus infection of lymphocytes, macrophages and dendritic cells

Journal of General Virology - Tập 89 Số 1 - Trang 277-287 - 2008
F. Reggeti1, Cameron Ackerley2, Dorothee Bienzle1
1Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada
2The Hospital for Sick Children, Toronto, ON M5G 1X8, Canada

Tóm tắt

The lymphotropic lentiviruses feline immunodeficiency virus (FIV) and human immunodeficiency virus (HIV) enter cells by sequential interaction with primary receptors CD134 or CD4, respectively, and subsequently with chemokine receptors. The host-cell range for FIV is broader than that for HIV, but whether this is a function of receptor expression is unknown. Lack of reagents specific to feline molecules has limited detection and analysis of receptors and their interaction with viral components. Here, the expression of CD134 and CXCR4 on feline T and B lymphocytes, dendritic cells (DCs) and macrophages was examined and the kinetics of FIV replication were assessed. Quantification of CD134 mRNA by real-time PCR indicated expression in all leukocytes, with significantly more transcripts in CD4+lymphocytes than in other leukocytes. Antibodies against human CD134 bound inconsistently to feline leukocytes. CXCR4 was detected with antibody clone 12G5 on the surface of monocyte-derived cells only, but gene transcripts were present in all cells, with the highest copy number in lymphocytes. CXCR4 expression decreased and CD134 expression increased with cell activation in lymphocytes. A subtype B biological isolate of FIV infected DCs, macrophages and lymphocytes, with the highest replication in CD4+lymphocytes, whilst cloned FIV P14 infected all cells, but replicated less efficiently. Although viral replication was lower in DCs and macrophages than in lymphocytes, DCs expressed specific receptors and were infected productively with FIV, as indicated by viral ultrastructure and DNA detection. These results may implicate altered function of DCs in the induction of specific immunity against FIV.

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Tài liệu tham khảo

al Shamkhani, 1996, OX40 is differentially expressed on activated rat and mouse T cells and is the sole receptor for the OX40 ligand, Eur J Immunol, 26, 1695, 10.1002/eji.1830260805

Bachmann, 1997, Genetic diversity of feline immunodeficiency virus: dual infection, recombination, and distinct evolutionary rates among envelope sequence clades, J Virol, 71, 4241, 10.1128/JVI.71.6.4241-4253.1997

Baribaud, 2001, Antigenically distinct conformations of CXCR4, J Virol, 75, 8957, 10.1128/JVI.75.19.8957-8967.2001

Bendinelli, 1995, Feline immunodeficiency virus: an interesting model for AIDS studies and an important cat pathogen, Clin Microbiol Rev, 8, 87, 10.1128/CMR.8.1.87

Bendinelli, 2001, During readaptation in vivo, a tissue culture-adapted strain of feline immunodeficiency virus reverts to broad neutralization resistance at different times in individual hosts but through changes at the same position of the surface glycoprotein, J Virol, 75, 4584, 10.1128/JVI.75.10.4584-4593.2001

Bienzle, 2003, Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow, Vet Immunol Immunopathol, 96, 19, 10.1016/S0165-2427(03)00132-6

Brelot, 1997, Role of the first and third extracellular domains of CXCR-4 in human immunodeficiency virus coreceptor activity, J Virol, 71, 4744, 10.1128/JVI.71.6.4744-4751.1997

Brelot, 1999, Effect of mutations in the second extracellular loop of CXCR4 on its utilization by human and feline immunodeficiency viruses, J Virol, 73, 2576, 10.1128/JVI.73.4.2576-2586.1999

Brunner, 1989, Infection of peritoneal macrophages in vitro and in vivo with feline immunodeficiency virus, J Virol, 63, 5483, 10.1128/JVI.63.12.5483-5488.1989

Dean, 1996, Proviral burden and infection kinetics of feline immunodeficiency virus in lymphocyte subsets of blood and lymph node, J Virol, 70, 5165, 10.1128/JVI.70.8.5165-5169.1996

Dean, 1999, Differential cell tropism of feline immunodeficiency virus molecular clones in vivo, J Virol, 73, 2596, 10.1128/JVI.73.4.2596-2603.1999

de Parseval, 2004a, Feline immunodeficiency virus targets activated CD4+ T cells by using CD134 as a binding receptor, Proc Natl Acad Sci U S A, 101, 13044, 10.1073/pnas.0404006101

de Parseval, 2004b, Factors that increase the effective concentration of CXCR4 dictate feline immunodeficiency virus tropism and kinetics of replication, J Virol, 78, 9132, 10.1128/JVI.78.17.9132-9143.2004

de Parseval, 2004c, Specific interaction of feline immunodeficiency virus surface glycoprotein with human DC-SIGN, J Virol, 78, 2597, 10.1128/JVI.78.5.2597-2600.2004

de Parseval, 2005, Structural mapping of CD134 residues critical for interaction with feline immunodeficiency virus, Nat Struct Mol Biol, 12, 60, 10.1038/nsmb872

de Parseval, 2006, Sequential CD134-CXCR4 interactions in feline immunodeficiency virus (FIV): soluble CD134 activates FIV Env for CXCR4-dependent entry and reveals a cryptic neutralization epitope, J Virol, 80, 3088, 10.1128/JVI.80.6.3088-3091.2006

Doms, 2000, Beyond receptor expression: the influence of receptor conformation, density, and affinity in HIV-1 infection, Virology, 276, 229, 10.1006/viro.2000.0612

Dow, 1999, In vivo monocyte tropism of pathogenic feline immunodeficiency viruses, J Virol, 73, 6852, 10.1128/JVI.73.8.6852-6861.1999

English, 1993, In vivo lymphocyte tropism of feline immunodeficiency virus, J Virol, 67, 5175, 10.1128/JVI.67.9.5175-5186.1993

Everitt, 1995, The analysis of repeated measures: a practical review with examples, Statistician, 44, 113, 10.2307/2348622

Farzan, 2002, The role of post-translational modifications of the CXCR4 amino terminus in stromal-derived factor 1 α association and HIV-1 entry, J Biol Chem, 277, 29484, 10.1074/jbc.M203361200

Frank, 2002, Infectious and whole inactivated simian immunodeficiency viruses interact similarly with primate dendritic cells (DCs): differential intracellular fate of virions in mature and immature DCs, J Virol, 76, 2936, 10.1128/JVI.76.6.2936-2951.2002

Gramaglia, 1998, Ox-40 ligand: a potent costimulatory molecule for sustaining primary CD4 T cell responses, J Immunol, 161, 6510, 10.4049/jimmunol.161.12.6510

Hernandez, 1996, Virus-cell and cell-cell fusion, Annu Rev Cell Dev Biol, 12, 627, 10.1146/annurev.cellbio.12.1.627

Hurtrel, 1994, Early events in lymph nodes during infection with SIV and FIV, Res Virol, 145, 221, 10.1016/S0923-2516(07)80026-4

Lane, 1999, Quantitative HIV culture, HIV Protocols, 11, 10.1385/0-89603-369-4:11

Lapham, 2002, CXCR4 heterogeneity in primary cells: possible role of ubiquitination, J Leukoc Biol, 72, 1206, 10.1189/jlb.72.6.1206

Lekkerkerker, 2006, Viral piracy: HIV-1 targets dendritic cells for transmission, Curr HIV Res, 4, 169, 10.2174/157016206776055020

Olmsted, 1989, Molecular cloning of feline immunodeficiency virus, Proc Natl Acad Sci U S A, 86, 2448, 10.1073/pnas.86.7.2448

Paterson, 1987, Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts, Mol Immunol, 24, 1281, 10.1016/0161-5890(87)90122-2

Patterson, 1995, CD4 expression on dendritic cells and their infection by human immunodeficiency virus, J Gen Virol, 76, 1155, 10.1099/0022-1317-76-5-1155

Phillips, 1990, Comparison of two host cell range variants of feline immunodeficiency virus, J Virol, 64, 4605, 10.1128/JVI.64.10.4605-4613.1990

Pion, 2007, Analysis of HIV-1-X4 fusion with immature dendritic cells identifies a specific restriction that is independent of CXCR4 levels, J Invest Dermatol, 127, 319, 10.1038/sj.jid.5700518

Reggeti, 2004, Feline immunodeficiency virus subtypes A, B and C and intersubtype recombinants in Ontario, Canada, J Gen Virol, 85, 1843, 10.1099/vir.0.19743-0

Shimojima, 2004, Use of CD134 as a primary receptor by the feline immunodeficiency virus, Science, 303, 1192, 10.1126/science.1092124

Sloane, 2005, Marked structural and functional heterogeneity in CXCR4: separation of HIV-1 and SDF-1 α responses, Immunol Cell Biol, 83, 129, 10.1111/j.1440-1711.2004.01304.x

Sodora, 1994, Identification of three feline immunodeficiency virus (FIV) env gene subtypes and comparison of the FIV and human immunodeficiency virus type 1 evolutionary patterns, J Virol, 68, 2230, 10.1128/JVI.68.4.2230-2238.1994

Steinman, 2006, Dendritic cells: translating innate to adaptive immunity, Curr Top Microbiol Immunol, 311, 17

Talbott, 1989, Nucleotide sequence and genomic organization of feline immunodeficiency virus, Proc Natl Acad Sci U S A, 86, 5743, 10.1073/pnas.86.15.5743

Toyosaki, 1993, Localization of the viral antigen of feline immunodeficiency virus in the lymph nodes of cats at the early stage of infection, Arch Virol, 131, 335, 10.1007/BF01378636

van der Meer, 2007, Feline immunodeficiency virus infection is enhanced by feline bone marrow-derived dendritic cells, J Gen Virol, 88, 251, 10.1099/vir.0.82450-0

Willett, 1997a, Common mechanism of infection by lentiviruses, Nature, 385, 587, 10.1038/385587a0

Willett, 1997b, Shared usage of the chemokine receptor CXCR4 by the feline and human immunodeficiency viruses, J Virol, 71, 6407, 10.1128/JVI.71.9.6407-6415.1997

Willett, 2003, Expression of CXCR4 on feline peripheral blood mononuclear cells: effect of feline immunodeficiency virus infection, J Virol, 77, 709, 10.1128/JVI.77.1.709-712.2003

Willett, 2006, Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus, J Virol, 80, 7744, 10.1128/JVI.00722-06

Zaitseva, 2005, Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association, J Leukoc Biol, 78, 1306, 10.1189/jlb.0105043

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Journal of General Virology

Tập 89 Số 1

277-287

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