Broad-range potential of Asphodelus microcarpus leaves extract for drug development
Tóm tắt
Many plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities. In order to find novel antimicrobial and antiviral agents, the aim of the present study was the evaluation of the antibacterial and antibiofilm susceptibility of Asphodelus microcarpus leaves extract. Moreover, the antiviral activity and the phytochemical composition of the active extract were also determined. Antimicrobial and antibiofilm activities of leaves ethanol extract of A. microcarpus were evaluated on 13 different microbial strains. We selected three different sets of microorganisms: (i) Gram-positive bacteria, (ii) Gram-negative bacteria and (iii) yeasts. The potential antiviral activity of A. microcarpus leaves ethanol extract was evaluated with a luciferase reporter gene assay in which the dsRNA-dependent RIG-I-mediated IFN-β activation was inducted or inhibited by the Ebola virus VP35 protein. HPLC-DAD-MS was used to identify phenolic profile of the active extract.
A. microcarpus leaves extract showed a potent inhibitory activity on Gram-positive bacteria while only a reduced inhibition was observed on Gram-negative bacteria. No activity was detected against Yeasts. The extract also showed an interesting antibiofilm motif on various bacterial strains (E. coli, S. aureus, S. haemolyticus and B. clausii). Moreover, this extract significantly affected the Ebola virus VP35 inhibition of the viral RNA (vRNA) induced IFN response. The overall results provide supportive data on the use of A. microcarpus as antimicrobial agent and a potential source of anti-viral natural products. Data collected set the bases for further studies for the identification of single active components and the development of new pharmaceuticals.
Tài liệu tham khảo
Sharma SB, Gupta R. Drug development from natural resource: a systematic approach. Mini-Rev Med Chem. 2015;15:52–7.
Fang J, Liu C, Wang Q, Lin P, Cheng F. In silico polypharmacology of natural products. Brief Bioinform. 2017;1–19. doi: 10.1093/bib/bbx045.
Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharmacol. 2000;32:S81–118.
Cowan MM. Plant products as anti-microbialagents. Clin Microbiol Rev. 1999;12:564–82.
Abdollahzadeh S, Mashouf R, Mortazavi H, Moghaddam M, Roozbahani N, Vahedi M. Antibacterial and antifungal activities of Punica granatum peel extracts against oral pathogens. J Dent (Tehran). 2011;8(1):1–6.
Pisano MB, Cosentino S, Viale S, Spanò D, Corona A, Esposito F, Tramontano E, Montoro P, Tuberoso CIG, Medda R, Pintus F. Biological activities of aerial parts extracts of Euphorbia characias. Biomed Res Int. 2016;2016:1–11.
Mocan A, Crișan G, Vlase L, Crișan O, Dan Cristian Vodnar AA, Raita O, Gheldiu A-M, Toiu A, Oprean R, Tilea I. Comparative studies on Polyphenolic composition, antioxidant and antimicrobial activities of Schisandra chinensis leaves and fruits. Molecules. 2014;19(9):15162–79.
Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science. 1999;21(284(5418)):1318–22.
Mah TF, O'Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 2001;9(1):34–9.
Lieberman D, Lieberman D. Pseudomonal infections in patients with COPD: epidemiology and management. Am J Respir Med. 2003;2(6):459–68.
Kumar S, Pandey AK. Chemistry and Biological Activities of Flavonoids: An Overview. ScientificWorldJournal. 2013;2013. Article ID 162750. 16 pages.
Pintus F, Spanò D, Corona A, Medda R. Antityrosinase activity of Euphorbia characias extracts. PeerJ. 2015;vol 3, article e1305. doi: 10.7717/peerj.1305.
Rosa A, Maxia A, Putzu D, Atzeri A, Era B, Fais A, Sanna C, Piras A. Chemical composition of Lycium Europaeum fruit oil obtained by supercritical CO2 extraction and evaluation of its antioxidant activity, cytotoxicity and cell absorption. Food Chem. 2017;230:82–90.
Rashed R, Medda R, Spanò D, Pintus F. Evaluation of antioxidant, anti-tyrosinase potentials and phytochemical composition of four Egyptian plants. Int Food Res J. 2016;23(1):203–10.
Wahyuni TS, Tumewu L, Permanasari AA, Apriani E, Adianti M, Rahman A, Widyawaruyanti A, Lusida MI, Fuad A, Fuchino H, Kawahara N, Shoji I, Deng L, Aoki C, Hotta H. Antiviral activities of Indonesian medicinal plants in the east java region against hepatitis C virus. Virol J. 2013;259:1–9. doi:10.1186/1743-422X-10-259.
Esposito F, Corona C, Zinzula L, Kharlamova T, Tramontano E. New Anthraquinone Derivates as Inhibitors of the HIV-1 Reverse transcriptase-Associated Ribonuclease H Function. Chemotherapy. 2012;58:299–307. doi:10.1159/000343101.
Derksen A, Kühn J, Hafezi W, Sendker J, Ehrhardt C, Ludwig S, Hensel A. Antiviral activity of hydroalcoholic extract from Eupatorium perfoliatum L. against the attachment of influenza a virus. J Ethnopharmacol. 2016;188:144–52. doi:10.1016/j.jep.2016.05.016.
Bicchi C, Rubiolo P, Ballero M, Sanna C, Matteodo M, Esposito F, Zinzula L, Tramontano E. HIV-1-inhibiting activity of the essential oil of ridolfia segetum and oenanthe crocata. Planta Med. 2009;75:1331–5. doi:10.1055/s-0029-1185546.
Esposito F, Sanna C, Del Vecchio C, Cannas V, Venditti A, Corona A, Bianco A, Serrilli AM, Guarcini L, Parolin C, Ballero M, Tramontano E. Hypericum hircinum L. components as new single-molecule inhibitors of both HIV-1 reverse transcriptase-associated DNA polymerase and ribonuclease H activities. Pathog Dis. 2013;68:116–24. doi:10.1111/2049-632X.12051.
Xu L, Grandi N, Del Vecchio C, Mandas D, Corona A, Piano D, Esposito F, Parolin C, Tramontano E. From the traditional Chinese medicine plant Schisandra Chinensis new scaffolds effective on HIV-1 reverse transcriptase resistant to non-nucleoside inhibitors. J Microbiol. 2015;53:288–93. doi:10.1007/s12275-015-4652-0.
Haller O, Kochs G, Weber F. The interferon response circuit : induction and suppression by pathogenic viruses. Virology. 2006;344:119–30. doi:10.1016/j.virol.2005.09.024.
Ma DY, Suthar MS. Mechanisms of innate immune evasion in re-emerging RNA viruses. Curr Opin Virol. 2015;12:26–37. doi:10.1016/j.coviro.2015.02.005.
Zinzula L, Tramontano E. Strategies of highly pathogenic RNA viruses to block dsRNA detection by RIG-I-like receptors: Hide, mask, hit. Antivir Res. 2013;100(3):615–35. doi:10.1016/j.antiviral.2013.10.002.
Zinzula L, Esposito F, Pala D, Tramontano E. dsRNA binding characterization of full length recombinant wild type and mutants Zaire Ebolavirus VP35. Antivir Res. 2012;93(3):354–63.
Bruni A, Ballero M, Poli F. Quantitative ethnopharmacological study of the Campidano Valley and Urzulei district, Sardinia, Italy. J Ethnopharmacol. 1997;57:97–124.
Schäfer G, Kaschula CH. The Immunomodulation and anti-inflammatory effects of garlic Organosulfur compounds in cancer chemoprevention. Anti-Cancer Agents Medicinal Chemistry. 2014;14:233–40.
Täckholm V. Students Flora of Egypt. 2nd ed. Cairo: Cairo University Press; 1974. p. 629–30.
Sarri M, Mouyet FZ, Benziane M, Cheriet A. Traditional use of medicinal plants in a city at steppic character (M’sila, Algeria). J Pharm Pharmacognosy Res. 2014;2:31–5.
Loi MC, Maxia L, Maxia A. Ethnobotanical comparison between the villages of Escolca and Lotzorai (Sardinia, Italy). J Herbs Spices Medecinal Plants. 2005;11:67–84.
El-Seedi HR. Antimicrobial Arylcoumarins from Asphodelus Microcarpus. J Nat Prod. 2007;70:118–20.
Ghoneim MM, Ma G, El-Hela AA, Mohammad AE, Kottob S, ElGhaly S, Cutler SJ, Ross SA. Biologically active secondary metabolites fromAsphodelus microcarpus. Nat Prod Commun. 2013;8:1117–9.
Ghoneim MM, Elokely KM, El-Hela AA, Mohammad AE, Jacob M, Radwan MM, Doerksen RJ, Cutler SJ, Ross SA. Asphodosides A-E, anti-MRSA metabolites from Asphodelus Microcarpus. Phytochemistry. 2014;105:79–84.
Nelson K, Lyles JT, Li T, Saitta A, Addie-Noye E, Tyler P and Quave CL. Anti-Acne Activity of Italian Medicinal Plants Used for Skin Infection. Front Pharmacol. 2016;7:Article425.
Al-Kayali R, Kitaz A, Haroun M. Antibacterial activity of Asphodelin lutea and Asphodelus Microcarpus against Methicillin resistant Staphylococcus aureus isolates. Int J Pharmacognosy Phytochem Res. 2016;8(12):1964–8.
El-Ghaly E-SM. Phytochemical and biological activities of Asphodelus microcarpus leaves. J Pharmacognosy Phytochemistry. 2017;6(2):259–64.
Orrù G, Del Nero S, Tuveri E, Ciusa ML, Pilia F, Erriu M, Orrù G, Liciardi M, Piras V, Denotti G. Evaluation of antimicrobial-Antibiofilm activity of a hydrogen peroxide decontaminating system used in dental unit water lines. Open Dent J. 2010;4:140–6.
Cannas V, Daino GL, Corona A, Esposito F, Tramontano E. A Luciferase reporter gene assay to measure Ebola virus viral protein 35-associated inhibition of double-stranded RNA-stimulated, retinoic acid-inducible gene 1-mediated induction of interferon β. J Infect Dis. 2015;212:S277–81. doi:10.1093/infdis/jiv214.
Zhang X, Ao Z, Bello A, Ran X, Liu S, Wigle J, Kobinger G, Yao X. Characterization of the inhibitory effect of an extract of Prunella vulgaris on Ebola virus glycoprotein (GP)-mediated virus entry and infection. Antivir Res. 2016;127:20–31.
Sepandj F, Ceri H, Gibb A, Read R, Olson M. Minimum inhibitory concentration (MIC) versus minimum biofilm eliminating concentration (MBEC) in evaluation of antibiotic sensitivity of gram-negative bacilli causing peritonitis. Perit Dial Int. 2004;24(1):65–7.
Langfield RD, Scarano FJ, Heitzman ME, Kondo M, Hammond GB, Neto CC. Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia Galioides. J Ethnopharmacol. 2004;94(2–3):279–81.
Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008;3(2):163–75.
Merritt JH, Kadouri DE, and O'Toole GA. Growing and analyzing static biofilms. Curr Protoc Microbiol. 2005. doi: 10.1002/9780471729259.mc01b01s00.
Di Petrillo A, González-Paramás AM, Era B, Medda R, Pintus F, Santos-Buelga C, Fais A. Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts. BMC Complement Altern Med. 2016;16(1):453. doi:10.1186/s12906-016-1442-0.
Zhu J, Kaufmann GF. Quo vadis quorum quenching? Curr Opin Pharmacol. 2013;13(5):688–98.
Tarahovsky YS, Kim YA, Yagolnik EA, Muzafarov EN. Flavonoid-membrane interactions: involvement of flavonoid-metal complexes in raft signaling. Biochim Biophys Acta. 2014;1838(5):1235–46. doi:10.1016/j.bbamem.2014.01.021.
Joung DK, Lee YS, Han SH, Lee SW, Cha SW, Mun SH, Kong R, Kang OH, Song HJ, Shin DW, Kwon DY. Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus. Asian Pac J Trop Med. 2016;9(1):19–22. doi:10.1016/j.apjtm.2015.12.004.
Qiu J, Li H, Meng H, Hu C, Li J, Luo M, Dong J, Wang X, Wang J, Deng Y, Deng X. Impact of luteolin on the production of alpha-toxin by Staphylococcus aureus. Lett Appl Microbiol. 2011;53(2):238–43. doi:10.1111/j.1472-765X.2011.03098.x.
Luthra P, Aguirre S, Yen BC, Pietzsch CA, Sanchez-Aparicio MT, Tigabu B, Morlock LK, García-Sastre A, Leung DW, Williams NS, Fernandez-Sesma A, Bukreyev A, Basler CF. Topoisomerase II inhibitors induce DNA damage-dependent interferon responses circumventing Ebola virus immune evasion. MBio 2017;8:e00368-e00317. doi.org/10.1128/mBio.00368-17.
Bedard KM, Wang ML, Proll SC, Loo Y-M, Katze MG, Gale M, Iadonato SP. Isoflavone agonists of IRF-3 dependent Signaling have antiviral activity against RNA viruses. J Virol. 2012;86:7334–44. doi:10.1128/JVI.06867-11.
Kanadaswami C, Lee LT, Lee PPH, Hwang JJ, Ke FC, Huang YT, Lee MT. The antitumor activities of flavonoids. In Vivo (Brooklyn). 2005;19:895–910.
Seelinger G, Merfort I, Schempp CM. Anti-oxidant, anti-inflammatory and anti-allergic activities of luteolin. Planta Med. 2008;74:1667–77. doi:10.1055/s-0028-1088314.
Jeong E, Lee JY. Intrinsic and extrinsic regulation of innate immune receptors. Yonsei Med J. 2011;52(3):379–92. doi:10.3349/ymj.2011.52.3.379.