Bringing light to the inner life of synapses

e-Neuroforum - Tập 17 Số 4 - Trang 95-101 - 2011
Stephan J. Sigrist1, Carolin Wichmann2
1Genetics, Institute for Biology, Free University Berlin, Takustr. 6, 14195 Berlin, Germany
2NeuroCure Cluster of Excellence, Charité Berlin, Berlin, Germany

Tóm tắt

Abstract Chemical synapses are pivotal for information transfer and storage within neuronal circuit­y. At the same time, various diseases of the nervous system most likely originate from disturbances in synapse structure and function. Synapses are very fast, extremely con­trolled and effective communication devices, with synaptic vesicles fusing at specialized membrane domains associated with highly-ordered protein architectures (cytomatrices) traditionally seen using electron microsco­py. Drosophila synapses with prominent cytomatrices called T-bars provide per se a highly suitable model system to apply genetic analysis to the roles of these protein architectures. Here we describe the principles behind these techniques as well as their application to the analysis of the molecular architecture of the synapse. In this context, the advent of super-resolution light microscopy methods yielding two- to 10-fold higher resolution than conventional microscopy has provided an efficient tool.

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e-Neuroforum

Tập 17 Số 4

95-101

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