Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride

Blood - Tập 26 Số 2 - Trang 215-219 - 1965
Leonard S. Kaplow1,2,3, Charlotte O. Ladd3
1Veterans Administration Hospital, West Haven, Conn.
2Veterans Administration Hospital, West Haven, Conn.; Assistant Clinical Professor of Pathology, School of Medicine, Yale Uniuersity, New Haven, Conn.
3Veterans Administration Hospital, West Haven, Connecticut and the Department of Pathology, School of Medicine, Yale University.

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Abstract A method is described for demonstrating leukocyte peroxidase activity in which benzidine dihydrochloride is used as the indicator compound instead of the more commonly used but potentially more hazardous benzidine base. The method is highly sensitive and rapid and permits the use of fixed blood smears and organ imprints. The incubation mixture, which incorporates safranin as a counterstain, may be used over and over again, for as long as 6 months. The method is also applicable to fresh frozen tissue sections.

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Blood

Tập 26 Số 2

215-219

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