Bone as an ion exchange system: evidence for a link between mechanotransduction and metabolic needs

American Journal of Physiology - Endocrinology and Metabolism - Tập 282 Số 4 - Trang E851-E864 - 2002
Alessandro Rubinacci1, M. Covini2, C. Bisogni2, Isabella Villa1, Manuela Galli2, Carla Palumbo3, Marzia Ferretti3, Maria Antonietta Muglia3, Gastone Marotti3
1Bone Metabolic Unit, Scientific Institute H San Raffaele, 20132 Milano;
2Department of Bioengineering, Politecnico of Milano, 20133 Milano; and
3Department of Morphological Sciences Human Anatomy, University of Modena, 41100 Modena, Italy

Tóm tắt

To detect whether the mutual interaction occurring between the osteocytes-bone lining cells system (OBLCS) and the bone extracellular fluid (BECF) is affected by load through a modification of the BECF-extracellular fluid (ECF; systemic extracellular fluid) gradient, mice metatarsal bones immersed in ECF were subjected ex vivo to a 2-min cyclic axial load of different amplitudes and frequencies. The electric (ionic) currents at the bone surface were measured by a vibrating probe after having exposed BECF to ECF through a transcortical hole. The application of different loads and different frequencies increased the ionic current in a dose-dependent manner. The postload current density subsequently decayed following an exponential pattern. Postload increment's amplitude and decay were dependent on bone viability. Dummy and static loads did not induce current density modifications. Because BECF is perturbed by loading, it is conceivable that OBLCS tends to restore BECF preload conditions by controlling ion fluxes at the bone-plasma interface to fulfill metabolic needs. Because the electric current reflects the integrated activity of OBLCS, its evaluation in transgenic mice engineered to possess genetic lesions in channels or matrix constituents could be helpful in the characterization of the mechanical and metabolic functions of bone.

Từ khóa


Tài liệu tham khảo

10.1002/jbmr.5650101002

10.1126/science.6740320

10.1096/fasebj.13.9001.s101

10.1152/ajpendo.1989.256.1.E152

Canas F, 1969, Am J Physiol, 217, 117, 10.1152/ajplegacy.1969.217.1.117

10.1007/BF00370609

10.1152/ajprenal.1994.267.6.F909

10.1007/BF00302070

10.3181/00379727-130-33618

Green J., 1994, Miner Electrolyte Metab, 20, 7

10.3109/15368379309012862

10.1016/0021-9290(96)84536-2

10.1359/jbmr.2000.15.8.1620

10.1007/BF02238545

Marotti G., 2000, J Musculoskel Neuron Interact, 1, 133

10.1016/8756-3282(92)90452-3

10.1007/BF00582171

10.1007/s007740050065

10.1002/ar.1091980204

10.1007/BF02555122

Neuman WF., 1969, Fed Proc, 28, 1846

10.1007/BF02411220

10.1007/s004290050145

Palumbo C, 2001, J Musculoskel Neuron Interact, 1, 327

10.1016/8756-3282(90)90134-K

10.1159/000146907

Parfitt AM., 1987, Bone, 8, S1, 10.1016/8756-3282(87)90124-4

10.1007/BF02554879

10.1016/0034-5687(75)90053-5

10.1016/S8756-3282(96)00260-8

10.1007/BF02509442

10.1152/ajpendo.2000.278.1.E15

10.1007/s002239900536

10.1007/BF02546403

10.1007/BF02408535

10.1096/fasebj.8.11.8070637

10.1002/1097-4644(20001215)79:4<648::AID-JCB130>3.0.CO;2-Q

10.1002/jbmr.5650071404

Thông tin
Thông tin xuất bản

American Journal of Physiology - Endocrinology and Metabolism

Tập 282 Số 4

E851-E864

Thông tin tác giả