Blueprints for Biosensors: Design, Limitations, and Applications

Genes - Tập 9 Số 8 - Trang 375
Alex C. Carpenter1,2, Ian T. Paulsen2, Thomas C. Williams1,2
1CSIRO Synthetic Biology Future Science Platform, Canberra, ACT 2601, Australia
2Department of Molecular Sciences, Macquarie University, Sydney NSW 2109, Australia

Tóm tắt

Biosensors are enabling major advances in the field of analytics that are both facilitating and being facilitated by advances in synthetic biology. The ability of biosensors to rapidly and specifically detect a wide range of molecules makes them highly relevant to a range of industrial, medical, ecological, and scientific applications. Approaches to biosensor design are as diverse as their applications, with major biosensor classes including nucleic acids, proteins, and transcription factors. Each of these biosensor types has advantages and limitations based on the intended application, and the parameters that are required for optimal performance. Specifically, the choice of biosensor design must consider factors such as the ligand specificity, sensitivity, dynamic range, functional range, mode of output, time of activation, ease of use, and ease of engineering. This review discusses the rationale for designing the major classes of biosensor in the context of their limitations and assesses their suitability to different areas of biotechnological application.

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Tài liệu tham khảo

Way, 2014, Integrating biological redesign: Where synthetic biology came from and where it needs to go, Cell, 157, 151, 10.1016/j.cell.2014.02.039

Chao, 2017, Engineering biological systems using automated biofoundries, Metab. Eng., 42, 98, 10.1016/j.ymben.2017.06.003

Hughes, R.A., and Ellington, A.D. (2017). Synthetic DNA synthesis and assembly: Putting the synthetic in synthetic biology. Cold Spring Harb. Perspect. Biol., 9.

Marcellin, 2018, Advances in analytical tools for high throughput strain engineering, Curr. Opin. Biotechnol., 54, 33, 10.1016/j.copbio.2018.01.027

Turner, 2013, Biosensors: Sense and sensibility, Chem. Soc. Rev., 42, 3184, 10.1039/c3cs35528d

Tian, 2009, Imaging neural activity in worms, flies and mice with improved GAaMP calcium indicators, Nat. Methods, 6, 875, 10.1038/nmeth.1398

Xiao, 2005, Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor, Angew. Chem. Int. Ed. Engl., 44, 5456, 10.1002/anie.200500989

Scognamiglio, 2014, Biosensing technology for sustainable food safety, TrAC Trends Anal. Chem., 62, 1, 10.1016/j.trac.2014.07.007

Venugopal, 2002, Biosensors in fish production and quality control, Biosens. Bioelectron., 17, 147, 10.1016/S0956-5663(01)00180-4

Verma, 2003, A disposable microbial based biosensor for quality control in milk, Biosens. Bioelectron., 18, 1219, 10.1016/S0956-5663(03)00085-X

Hesari, N., Kıratlı Yılmazçoban, N., Elzein, M., Alum, A., and Abbaszadegan, M. (2017). A strategy to establish a quality assurance/quality control plan for the application of biosensors for the detection of E. Coli in water. Biosensors, 7.

Orgel, 2008, Biosensor-based on-site explosives detection using aptamers as recognition elements, Anal. Bioanal. Chem., 391, 1793, 10.1007/s00216-008-2150-5

Dekker, 2017, Sense and sensitivity in bioprocessing—Detecting cellular metabolites with biosensors, Curr. Opin. Chem. Biol., 40, 31, 10.1016/j.cbpa.2017.05.014

Biechele, 2015, Sensor systems for bioprocess monitoring, Eng. Life Sci., 15, 469, 10.1002/elsc.201500014

Fang, 2015, Current and prospective methods for plant disease detection, Biosensors, 5, 537, 10.3390/bios5030537

Ahn, 2004, Disposable smart lab on a chip for point-of-care clinical diagnostics, Proc. IEEE, 92, 154, 10.1109/JPROC.2003.820548

Soper, 2006, Point-of-care biosensor systems for cancer diagnostics/prognostics, Biosens. Bioelectron., 21, 1932, 10.1016/j.bios.2006.01.006

Amano, 2005, Detection of influenza virus: Traditional approaches and development of biosensors, Anal. Bioanal. Chem., 381, 156, 10.1007/s00216-004-2927-0

Smith, 2008, A review of biosensors and biologically-inspired systems for explosives detection, Analyst, 133, 571, 10.1039/b717933m

Tegos, 2013, Biodefense: Trends and challenges in combating biological warfare agents, Virulence, 4, 740, 10.4161/viru.27170

Klenkar, 2008, A microarray chip for label-free detection of narcotics, Anal. Bioanal. Chem., 391, 1679, 10.1007/s00216-008-1839-9

Zhang, 2015, Graphene oxide-based optical biosensor functionalized with peptides for explosive detection, Biosens. Bioelectron., 68, 494, 10.1016/j.bios.2015.01.040

Klutz, 2016, Cost evaluation of antibody production processes in different operation modes, Chem. Eng. Sci., 141, 63, 10.1016/j.ces.2015.10.029

Williams, 2016, Synthetic evolution of metabolic productivity using biosensors, Trends Biotechnol., 34, 371, 10.1016/j.tibtech.2016.02.002

Mehrotra, 2016, Biosensors and their applications—A review, J. Oral Biol. Craniofac. Res., 6, 153, 10.1016/j.jobcr.2015.12.002

Zhang, 2015, Development of biosensors and their application in metabolic engineering, Curr. Opin. Chem. Biol., 28, 1, 10.1016/j.cbpa.2015.05.013

Kotula, 2014, Programmable bacteria detect and record an environmental signal in the mammalian gut, Proc. Natl. Acad. Sci. USA, 111, 4838, 10.1073/pnas.1321321111

Birkedal, 2015, Construction of a fuzzy and boolean logic gates based on DNA, Small, 11, 1811, 10.1002/smll.201402755

Casadaban, 1976, Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu, J. Mol. Biol., 104, 541, 10.1016/0022-2836(76)90119-4

Kenyon, 1980, DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli, Proc. Natl. Acad. Sci. USA, 77, 2819, 10.1073/pnas.77.5.2819

Karig, 2017, Cell-free synthetic biology for environmental sensing and remediation, Curr. Opin. Biotechnol., 45, 69, 10.1016/j.copbio.2017.01.010

Pardee, 2014, Paper-based synthetic gene networks, Cell, 159, 940, 10.1016/j.cell.2014.10.004

Jensen, 2017, Lighting up yeast cell factories by transcription factor-based biosensors, FEMS Yeast Res., 17, fox076

Williams, 2017, Positive-feedback, ratiometric biosensor expression improves high-throughput metabolite-producer screening efficiency in yeast, Synth. Biol., 2, ysw002, 10.1093/synbio/ysw002

Siedler, 2014, SoxR as a single-cell biosensor for NADPH consuming enzymes in Escherichia coli, ACS Synth. Biol., 3, 41, 10.1021/sb400110j

Zhang, 2016, Engineering an NADPH/NADP+ redox biosensor in yeast, ACS Synth. Biol., 5, 1546, 10.1021/acssynbio.6b00135

Leavitt, J.M., Wagner, J.M., Tu, C.C., Tong, A., Liu, Y., and Alper, H.S. (2017). Biosensor-enabled directed evolution to improve muconic acid production in Saccharomyces cerevisiae. Biotechnol. J., 12.

Zhang, 2012, Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids, Nat. Biotechnol., 30, 354, 10.1038/nbt.2149

Binder, 2012, A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level, Genome Biol., 13, R40, 10.1186/gb-2012-13-5-r40

Seok, 2018, Directed evolution of the 3-hydroxypropionic acid production pathway by engineering aldehyde dehydrogenase using a synthetic selection device, Metab. Eng., 47, 113, 10.1016/j.ymben.2018.03.009

Mahr, 2015, Biosensor-driven adaptive laboratory evolution of l-valine production in Corynebacterium glutamicum, Metab. Eng., 32, 184, 10.1016/j.ymben.2015.09.017

Johnson, 2017, Design and application of genetically-encoded malonyl-CoAa biosensors for metabolic engineering of microbial cell factories, Metab. Eng., 44, 253, 10.1016/j.ymben.2017.10.011

Kasey, 2018, Development of transcription factor-based designer macrolide biosensors for metabolic engineering and synthetic biology, ACS Synth. Biol., 7, 227, 10.1021/acssynbio.7b00287

Chen, 2018, Engineering tunable biosensors for monitoring putrescine in Escherichia coli, Biotechnol. Bioeng., 115, 1014, 10.1002/bit.26521

Ruiz, 2015, Transcription factor-based biosensors enlightened by the analyte, Front. Microbiol., 6, 648

Gallegos, 1997, Arac/XylS family of transcriptional regulators, Microbiol. Mol. Biol. Rev., 61, 393

Ramos, 2005, The TetR family of transcriptional repressors, Microbiol. Mol. Biol. Rev., 69, 326, 10.1128/MMBR.69.2.326-356.2005

Shi, 2017, Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae, Bioresour. Technol., 245, 1343, 10.1016/j.biortech.2017.06.114

Dahl, 2013, Engineering dynamic pathway regulation using stress-response promoters, Nat. Biotechnol., 31, 1039, 10.1038/nbt.2689

Dietrich, 2013, Transcription factor-based screens and synthetic selections for microbial small-molecule biosynthesis, ACS Synth. Biol., 2, 47, 10.1021/sb300091d

Kurth, 2008, Involvement of BmoR and BmoG in n-alkane metabolism in Ppseudomonas butanovora, Microbiology, 154, 139, 10.1099/mic.0.2007/012724-0

Wang, 2016, Design and engineering of intracellular-metabolite-sensing/regulation gene circuits in Saccharomyces cerevisiae, Biotechnol. Bioeng., 113, 206, 10.1002/bit.25676

Lutz, 1997, Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Res., 25, 1203, 10.1093/nar/25.6.1203

Chou, 2013, Programming adaptive control to evolve increased metabolite production, Nat. Commun., 4, 2595, 10.1038/ncomms3595

Cherf, 2015, Applications of yeast surface display for protein engineering, Methods Mol. Biol., 1319, 155, 10.1007/978-1-4939-2748-7_8

Pande, 2010, Phage display: Concept, innovations, applications and future, Biotechnol. Adv., 28, 849, 10.1016/j.biotechadv.2010.07.004

Belkin, 2010, Where microbiology meets microengineering: Design and applications of reporter bacteria, Nat. Rev. Microbiol., 8, 511, 10.1038/nrmicro2392

Polizzi, 2015, Genetically-encoded biosensors for monitoring cellular stress in bioprocessing, Curr. Opin. Biotechnol., 31, 50, 10.1016/j.copbio.2014.07.011

Mahr, 2016, Transcription factor-based biosensors in biotechnology: Current state and future prospects, Appl. Microbiol. Biotechnol., 100, 79, 10.1007/s00253-015-7090-3

Rogers, 2016, Biosensor-based engineering of biosynthetic pathways, Curr. Opin. Biotechnol., 42, 84, 10.1016/j.copbio.2016.03.005

Tuerk, 1990, Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase, Science, 249, 505, 10.1126/science.2200121

Ellington, 1990, In Vitro selection of RNA molecules that bind specific ligands, Nature, 346, 818, 10.1038/346818a0

Ellington, 1992, Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures, Nature, 355, 850, 10.1038/355850a0

Song, 2008, Aptamer-based biosensors, TrAC Trends Anal. Chem., 27, 108, 10.1016/j.trac.2007.12.004

McKeague, M., Velu, R., Hill, K., Bardóczy, V., Mészáros, T., and DeRosa, M. (2014). Selection and characterization of a novel DNA aptamer for label-free fluorescence biosensing of Ochratoxin A. Toxins, 6.

Yingfu, L., and Yi, L. (2009). Artificial functional nucleic acids: Aptamers, ribozymes, and deoxyribozymes identified by in vitro selection. Functional Nucleic Acids for Analytical Applications, Springer.

McKeague, 2012, Challenges and opportunities for small molecule aptamer development, J. Nucleic Acids, 2012, 20, 10.1155/2012/748913

Sefah, 2010, Development of DNA aptamers using cell-selex, Nat. Protoc., 5, 1169, 10.1038/nprot.2010.66

Ruscito, A., and DeRosa, M.C. (2016). Small-molecule binding aptamers: Selection strategies, characterization, and applications. Front. Chem., 4.

Michener, 2012, Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways, Metab. Eng., 14, 212, 10.1016/j.ymben.2011.09.004

Gong, S., Wang, Y., Wang, Z., and Zhang, W. (2017). Computational methods for modeling aptamers and designing riboswitches. Int. J. Mol. Sci., 18.

McKeague, 2015, Comprehensive analytical comparison of strategies used for small molecule aptamer evaluation, Anal. Chem., 87, 8608, 10.1021/acs.analchem.5b02102

Alsaafin, 2017, Functional nucleic acids as in vivo metabolite and ion biosensors, Biosens. Bioelectron., 94, 94, 10.1016/j.bios.2017.02.030

Findeiss, S., Etzel, M., Will, S., Morl, M., and Stadler, P.F. (2017). Design of artificial riboswitches as biosensors. Sensors, 17.

Hejazi, 2016, Riboswitches: From living biosensors to novel targets of antibiotics, Gene, 592, 244, 10.1016/j.gene.2016.07.035

Machtel, 2016, Emerging applications of riboswitches—from antibacterial targets to molecular tools, J. Appl. Genet., 57, 531, 10.1007/s13353-016-0341-x

Muranaka, 2009, Efficient design strategy for whole-cell and cell-free biosensors based on engineered riboswitches, Anal. Lett., 42, 108, 10.1080/00032710802568556

Paige, 2012, Fluorescence imaging of cellular metabolites with RNA, Science, 335, 1194, 10.1126/science.1218298

Kundert, K., Lucas, J.E., Watters, K.E., Fellmann, C., Ng, A.H., Heineike, B.M., Fitzsimmons, C.M., Oakes, B.L., Savage, D.F., and El-Samad, H. (2018). Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs. bioRxiv.

Gilbert, 2009, Adaptive ligand binding by the purine riboswitch in the recognition of guanine and adenine analogs, Structure, 17, 857, 10.1016/j.str.2009.04.009

Mironov, 2002, Sensing small molecules by nascent RNA: A mechanism to control transcription in bacteria, Cell, 111, 747, 10.1016/S0092-8674(02)01134-0

Thore, 2006, Structure of the eukaryotic thiamine pyrophosphate riboswitch with its regulatory ligand, Science, 312, 1208, 10.1126/science.1128451

Hallberg, 2017, Engineering and in vivo applications of riboswitches, Annu. Rev. Biochem., 86, 515, 10.1146/annurev-biochem-060815-014628

Palchetti, 2012, Electrochemical nanomaterial-based nucleic acid aptasensors, Anal. Bioanal. Chem., 402, 3103, 10.1007/s00216-012-5769-1

Crulhas, 2017, An electrochemical aptasensor for detection of bovine interferon gamma, Anal. Methods, 9, 4527, 10.1039/C7AY01313B

Meirinho, 2014, Development of an electrochemical aptasensor for the detection of human osteopontin, Procedia Eng., 87, 316, 10.1016/j.proeng.2014.11.671

Song, 2013, Imaging bacterial protein expression using genetically encoded RNA sensors, Nat. Methods, 10, 873, 10.1038/nmeth.2568

Stein, 2015, Synthetic protein switches: Design principles and applications, Trends Biotechnol., 33, 101, 10.1016/j.tibtech.2014.11.010

Ostermeier, 2000, Evolution of protein function by domain swapping, Adv. Protein Chem., 55, 29, 10.1016/S0065-3233(01)55002-0

Nagai, 2001, Circularly permuted green fluorescent proteins engineered to sense Ca2+, Proc. Natl. Acad. Sci. USA, 98, 3197, 10.1073/pnas.051636098

Yu, 2011, Circular permutation: A different way to engineer enzyme structure and function, Trends Biotechnol., 29, 18, 10.1016/j.tibtech.2010.10.004

Guntas, 2004, A molecular switch created by in vitro recombination of nonhomologous genes, Chem. Biol., 11, 1483, 10.1016/j.chembiol.2004.08.020

Baird, 1999, Circular permutation and receptor insertion within green fluorescent proteins, Proc. Natl. Acad. Sci. USA, 96, 11241, 10.1073/pnas.96.20.11241

Ikura, 1992, Solution structure of a calmodulin-target peptide complex by multidimensional NMR, Science, 256, 632, 10.1126/science.1585175

Guo, 2016, Engineered PQQ-glucose dehydrogenase as a universal biosensor platform, J. Am. Chem. Soc., 138, 10108, 10.1021/jacs.6b06342

Okuda, 2004, PQQ glucose dehydrogenase with novel electron transfer ability, Biochem. Biophys. Res. Commun., 314, 793, 10.1016/j.bbrc.2003.12.167

Igarashi, 2004, Molecular engineering of PQQGDH and its applications, Arch. Biochem. Biophys., 428, 52, 10.1016/j.abb.2004.06.001

Psoma, 2010, A novel enzyme entrapment in SU-8 microfabricated films for glucose micro-biosensors, Biosens. Bioelectron., 26, 1582, 10.1016/j.bios.2010.07.117

Guo, 2016, Engineering PQQ-glucose dehydrogenase into an allosteric electrochemical Ca2+ sensor, Chem. Commun., 52, 485, 10.1039/C5CC07824E

Huang, 2010, Rational conversion of affinity reagents into label-free sensors for peptide motifs by designed allostery, ACS Chem. Biol., 5, 273, 10.1021/cb900284c

Huang, 2009, Structural basis for exquisite specificity of affinity clamps, synthetic binding proteins generated through directed domain-interface evolution, J. Mol. Biol., 392, 1221, 10.1016/j.jmb.2009.07.067

Stein, 2014, Protease-based synthetic sensing and signal amplification, Proc. Natl. Acad. Sci. USA, 111, 15934, 10.1073/pnas.1405220111

Sharma, 2016, Antibodies and antibody-derived analytical biosensors, Essays Biochem., 60, 9, 10.1042/EBC20150002