Blue-light induced biosynthesis of ROS contributes to the signaling mechanism of Arabidopsis cryptochrome

Scientific Reports - Tập 7 Số 1
Mohamed A. El‐Esawi1, Louis-David Arthaut1, Nathalie Jourdan1, Alain d’Harlingue1, Justin Link2, Carlos F. Martino3, Margaret Ahmad2
1UMR CNRS 8256 (B2A), IBPS, Université Paris VI, Paris, 75005, France
2Department of Physics, Xavier University, Cincinnati, Ohio, 45207, USA
3Department of Biomedical Engineering, Florida Institute of Technology Melbourne, FL 32901, USA

Tóm tắt

AbstractCryptochromes are evolutionarily conserved blue light receptors with many roles throughout plant growth and development. They undergo conformational changes in response to light enabling interaction with multiple downstream signaling partners. Recently, it has been shown that cryptochromes also synthesize reactive oxygen species (ROS) in response to light, suggesting the possibility of an alternate signaling mechanism. Here we show by fluorescence imaging and microscopy that H202and ROS accumulate in the plant nucleus after cryptochrome activation. They induce ROS-regulated transcripts including for genes implicated in pathogen defense, biotic and abiotic stress. Mutant cryptochrome alleles that are non-functional in photomorphogenesis retain the capacity to induce ROS-responsive phenotypes. We conclude that nuclear biosynthesis of ROS by cryptochromes represents a new signaling paradigm that complements currently known mechanisms. This may lead to novel applications using blue light induced oxidative bursts to prime crop plants against the deleterious effects of environmental stresses and toxins.

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