Biostability of the Proanthocyanidins-Dentin Complex and Adhesion Studies

SAGE Publications - Tập 96 Số 4 - Trang 406-412 - 2017
Ariene A. Leme‐Kraus1, B. Aydın1, Cristina de Mattos Pimenta Vidal1, Rasika M Phansalkar2, Joo‐Won Nam2, James B. McAlpine2, Guido F. Pauli2, Shao‐Nong Chen2, Ana K. Bedran‐Russo1
1Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
2Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL USA

Tóm tắt

Oligomeric proanthocyanidins (OPACs) are potent and renewable natural bioactives possible to be refined into chemically standardized mixtures for biological applications. Herein, we found that multiscale interactions of OPACs with the dentin matrix create tight biointerfaces with hydrophobic methacrylate adhesives on wet surfaces. An enriched mixture of OPACs, with a known phytochemical profile, was produced from grape seed crude extract ( Vitis vinifera; enriched grape seed extract [e-GSE]) and applied to dentin matrices to determine changes to the mechanical properties and biodegradability of the dentin matrix and favorable resin adhesion mechanisms. Methods included a 3-point flexural test, quantification of hydroxyproline (collagen solubilization), static and dynamic nanomechanical analyses, resin-dentin microtensile bond strength, and micropermeability at the adhesive interface. The e-GSE-modified dentin matrix exhibited remarkably low collagen solubilization and sustained the bulk elastic properties over 12 mo. Tan δ findings reveal a more elastic-like behavior of the e-GSE-modified dentin matrix, which was not affected by H-bond destabilization by urea. Dentin-methacrylate biointerfaces with robust and stable adhesion were created on e-GSE-primed dentin surfaces, leading to a dramatic decrease of the interfacial permeability. Standardized OPAC mixtures provide a new mechanism of adhesion to type I collagen–rich tissues that does not rely on hydrophilic monomers. The bioadhesion mechanism involves physicochemical modifications to the dentin matrix, reduced tissue biodegradation, and bridging to methacrylate resins.

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Tài liệu tham khảo

10.1002/jbm.b.31417

American Dental Association. 2007. 2005–06 survey of dental services rendered. Chicago (IL): American Dental Association.

10.1016/j.jdent.2015.05.001

Aydin B, 2016, J Adhes Dent

10.1016/j.jsb.2008.02.010

10.1016/j.actbio.2010.12.013

10.1016/j.micron.2012.03.021

10.1016/j.actbio.2012.02.022

10.1016/j.jbiomech.2012.03.021

10.1155/2013/918010

10.1177/154405910808700704

10.1021/am400455p

10.1016/j.ijbiomac.2010.08.003

10.1002/jbm.a.10460

10.1016/S0109-5641(00)00035-X

10.3390/molecules13102674

10.1016/j.ijbiomac.2010.12.012

10.1021/am4009538

10.1111/j.1524-4725.1998.tb04074.x

Kim SJ, 1998, J Dermatol, 25, 85, 10.1111/j.1346-8138.1998.tb02355.x

10.1126/science.1147241

10.1016/j.jbiomech.2015.03.016

10.1126/science.aab0556

10.1177/0022034512439210

10.1002/jbm.b.31010

10.1016/j.dental.2015.09.001

10.1073/pnas.0502718103

10.1016/j.fitote.2014.12.006

10.1016/0009-9120(96)00003-6

10.1016/j.jbiomech.2012.11.017

10.1021/bm200464x

10.1016/j.dental.2010.07.006

10.1002/app.10262

10.1016/j.actbio.2014.03.036

10.1021/la5034383

10.1002/1097-4636(2001)58:1<47::AID-JBM70>3.0.CO;2-U

10.1016/j.biomaterials.2005.05.011

10.1016/j.biomaterials.2013.10.017

10.1002/(SICI)1097-0134(19980501)31:2<107::AID-PROT1>3.0.CO;2-J

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SAGE Publications

Tập 96 Số 4

406-412

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