Biomimetic Growth and Self‐Assembly of Fluorapatite Aggregates by Diffusion into Denatured Collagen Matrices
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Kuhn L. T., 1996, Biomimetic Materials Chemistry
Experimental Procedure: Pig skin gelatin (5.5 g 300 Bloom Aldrich) was placed in water (50 mL) acidified with glutamic acid or 2N HCl to pH 2.5–3.5 and then heated with stirring for a few minutes in a water bath. The central tube of the diffusion cell (length 3 cm diameter 2.5 cm closed at one end) was filled with the hot gelatin and left at room temperature for 24 h in a vertical position until gelation occurred. The L‐shaped tubes (vertical section: 18 cm horizontal section: 6 cm standard ground joints) were then attached to the central gelatin tube filled with the aqueous solutions (0.133M CaCl2or 0.08 M Na2HPO4/ 0.027 M KF) and closed with ground glass stoppers. Both solutions were previously adjusted to the physiological pH of 7.4 with α α α‐tris(hydroxymethyl)methylamine/HCI. The diffusion experiments were carried out at 25°C (thermostat). At the end of the experiment the gelatin plug was pressed out of the central tube and cut into sections corresponding to the Liesegang bands. To isolate the solids these sections were washed several times with hot water and centrifuged.
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