Biomaterial topography alters healing in vivo and monocyte/macrophage activation in vitro

Journal of Biomedical Materials Research - Part A - Tập 95A Số 2 - Trang 649-657 - 2010
Paige C. S. Bota1,2,3, Angela M. B. Collie4,1,3, Pauli Puolakkainen5,6, Robert B. Vernon6, E. Helene Sage6, Buddy D. Ratner1, Patrick S. Stayton1
1Department of Bioengineering, University of Washington, Seattle, Washington 98195
2Edwards Lifesciences, Irvine, CA 92614
3these authors contributed equally to this work
4Cleveland Clinic, Cleveland, OH 44195;
5Helsinki University Central Hospital, Helsinki, Finland and Turku University Central Hospital, Turku, Finland
6Hope Heart Program, Benaroya Research Institute at Virginia Mason, Seattle, Washington 98101

Tóm tắt

AbstractThe effect of biomaterial topography on healing in vivo and monocyte/macrophage stimulation in vitro was assessed. A series of expanded polytetrafluoroethylene (ePTFE) materials were characterized by increasing average intranodal distance of 1.2 μm (1.2‐ePTFE), 3.0 μm (3.0‐ePTFE), and 4.4 μm (4.4‐ePTFE), but presented consistent surface chemistry with nonporous PTFE (np‐PTFE). Subcutaneous implantation of 4.4‐ePTFE into mice resulted in a statistically thinner capsule that appeared less organized and less dense than the np‐PTFE response. In vitro, isolated monocytes/macrophages cultured on np‐PTFE produced low levels of interleukin 1‐beta (IL‐1β), 1.2‐ePTFE and 3.0‐ePTFE stimulated intermediate levels, and 4.4‐ePTFE stimulated a 15‐fold increase over np‐PTFE. Analysis of cDNA microarrays demonstrated that additional proinflammatory cytokines and chemokines, including IL‐1β, interleukin 6, tumor necrosis factor alpha, monocyte chemotactic protein 1, and macrophage inflammatory protein 1‐beta, were expressed at higher levels by monocytes/macrophages cultured on 4.4‐ePTFE at 4 and 24 h, respectively. Expression ratios for several genes were quantified by RT‐PCR and were consistent with those from the cDNA array results. These results demonstrate the effect of biomaterial topography on early proinflammatory cytokine production and gene transcription by monocytes/macrophages in vitro and decreased fibrous capsule thickness in vivo. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.

Từ khóa


Tài liệu tham khảo

10.1146/annurev.matsci.31.1.81

10.1016/0142-9612(88)90063-4

10.1002/jbm.a.10425

10.1097/00024382-200014040-00003

10.1002/jbm.820220805

10.1002/jbm.820290217

10.1002/jbm.a.30967

10.1002/jbm.a.31221

10.1163/156856295X00698

10.1006/excr.1996.0098

10.1002/jbm.820290112

10.3109/08941938909016503

10.1002/(SICI)1097-4636(19971205)37:3<401::AID-JBM11>3.0.CO;2-E

10.1002/(SICI)1097-4636(199603)30:3<305::AID-JBM5>3.0.CO;2-U

10.1002/jbm.820180407

10.1002/jbm.820260703

10.1002/jbm.820270910

10.1002/jbm.820230904

10.1002/jbm.820260702

10.1002/jbm.820291208

10.1002/(SICI)1097-4636(19980615)40:4<586::AID-JBM10>3.0.CO;2-E

10.1002/(SICI)1097-4636(19970315)34:4<463::AID-JBM7>3.0.CO;2-I

10.1128/MCB.21.19.6450-6460.2001

Rozen S, 2000, Bioinformatics Methods and Protocols: Methods in Molecular Biology, 365

Middleton E, 1998, Allergy: Principles and Practice

10.1056/NEJM199802123380706

10.1007/s00011-004-1298-5

10.1189/jlb.70.4.478

10.1006/cyto.1998.0426

10.1016/S0022-1759(02)00218-1

10.1016/S0166-0934(03)00191-5

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part A

Tập 95A Số 2

649-657

Thông tin tác giả