Biological synthesis of Au nanoparticles using liquefied mash of cassava starch and their functionalization for enhanced hydrolysis of xylan by recombinant xylanase

Bioprocess and Biosystems Engineering - Tập 39 - Trang 785-792 - 2016
Sumei Zeng1,2, Liangwei Du1,3, Meiying Huang1,3, Jia-Xun Feng1,2
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, People’s Republic of China
2College of Life Science and Technology, Guangxi University, Nanning, People’s Republic of China
3College of Chemistry and Chemical Engineering, Guangxi University, Nanning, People’s Republic of China

Tóm tắt

Au nanoparticles (AuNPs) have shown the potential for a variety of applications due to their unique physical and chemical properties. In this study, a facile and affordable method for the synthesis of AuNPs via the liquefied mash of cassava starch has been described and the functionalized AuNPs by l-cysteine improved activity of recombinant xylanase was demonstrated. UV–Vis absorption spectroscopy, transmission electron microscopy, and zeta potential measurements were performed to characterize the AuNPs and monitor their synthesis. The presence of Au was confirmed by energy-dispersive X-ray spectroscopy (EDX) and the X-ray diffraction patterns showed that Au nanocrystals were face-centered cubic. The C=O stretching vibration in the Fourier transform infrared spectrum of AuNPs suggested that the hemiacetal C–OH of sugar molecules performed the reduction of Au3+ to Au0. The presence of C and O in the EDX spectrum and the negative zeta potential of AuNPs suggested that the biomolecules present in liquefied cassava mash were responsible for the stabilization of AuNPs. The surface of AuNPs was easily functionalized by l-cysteine, which improved the stability of AuNPs. Moreover, cysteine-functionalized AuNPs could significantly improve recombinant xylanase efficiency and stability.

Tài liệu tham khảo

Lee KYJ, Wang Y, Nie S (2015) In vitro study of a pH-sensitive multifunctional doxorubicin-gold nanoparticle system: therapeutic effect and surface enhanced Raman scattering. RSC Adv 5:65651–65659 Deng D, Li Y, Xue J, Wang J, Ai G, Li X, Gu Y (2015) Gold nanoparticle-based beacon to detect STAT5b mRNA expression in living cells: a case optimized by bioinformatics screen. Int J Nanomed 10:3231–3244 Mihranyan A, Ferraz N, Strømme M (2012) Current status and future prospects of nanotechnology in cosmetics. Prog Mater Sci 57:875–910 Basavegowda N, Idhayadhulla A, Lee YR (2014) Preparation of Au and Ag nanoparticles using Artemisia annua and their in vitro antibacterial and tyrosinase inhibitory activities. Mater Sci Eng C-Mater 43:58–64 Garg S, De A, Mozumdar S (2015) pH-dependent immobilization of urease on glutathione-capped gold nanoparticles. J Biomed Mater Res 103:1771–1783 Shankar SS, Ahmad A, Pasricha R, Sastry M (2003) Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J Mater Chem 13:1822–1826 Andeani JK, Kazemi H, Mohsenzadeh S, Safavi A (2011) Biosynthesis of gold nanoparticles using dried flowers extract of Achillea wilhelmsii plant. Dig J Nanomater Bios 6:1011–1017 Gangula A, Podila R, Ramakrishna M, Karanam L, Janardhana C, Rao AM (2011) Catalytic reduction of 4-nitrophenol using biogenic gold and silver nanoparticles derived from Breynia rhamnoides. Langmuir 27:15268–15274 Basavegowda N, Idhayadhulla A, Lee YR (2014) Phyto-synthesis of gold nanoparticles using fruit extract of Hovenia dulcis and their biological activities. Ind Crop Prod 52:745–751 Jayaseelan C, Ramkumar R, Rahuman AA, Perumal P (2013) Green synthesis of gold nanoparticles using seed aqueous extract of Abelmoschus esculentus and its antifungal activity. Ind Crop Prod 45:423–429 Kumar CG, Mamidyala SK, Sreedhar B, Reddy BV (2011) Synthesis and characterization of gold glyconanoparticles functionalized with sugars of sweet sorghum syrup. Biotechnol Progr 27:1455–1463 Xia B, He F, Li L (2013) Preparation of bimetallic nanoparticles using a facile green synthesis method and their application. Langmuir 29:4901–4907 Philip D (2009) Honey mediated green synthesis of gold nanoparticles. Spectrochim Acta A 73:650–653 Maeda H, Kajiwara S, Araujo NQ (1982) The separation of solids from the liquefied mash of cassava tuber and continuous saccharification by immobilized glucoamylase. Eur J Appl Microbiol Biotechnol 16:92–98 Yang Q, Gao Y, Huang Y, Xu Q, Luo X-M, Liu J-L, Feng J-X (2015) Identification of three important amino acid residues of xylanase AfxynA from Aspergillus fumigatus for enzyme activity and formation of xylobiose as the major product. Process Biochem 50:571–581 Upadhyay LSB, Verma N (2014) Synthesis and characterization of cysteine functionalized silver nanoparticles for biomolecule immobilization. Bioprocess Biosys Eng 37:2139–2148 Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428 Mo XC, Chen CL, Pang H, Feng Y, Feng JX (2010) Identification and characterization of a novel xylanase derived from a rice straw degrading enrichment culture. Appl Microbiol Biot 87:2137–2146 Pal A, Khanum F (2011) Purification of xylanase from Aspergillus niger DFR-5: individual and interactive effect of temperature and pH on its stability. Process Biochem 46:879–887 Kasthuri J, Veerapandian S, Rajendiran N (2009) Biological synthesis of silver and gold nanoparticles using apiin as reducing agent. Colloid Surf B 68:55–60 Velázquez-Salazar JJ, Esparza R, Mejía-Rosales SJ, Estrada-Salas R, Ponce A, Deepak FL, Castro-Guerrero C, José-Yacamán M (2011) Experimental evidence of icosahedral and decahedral packing in one-dimensional nanostructures. ACS Nano 5:6272–6278 Song JY, Jang H-K, Kim BS (2009) Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts. Process Biochem 44:1133–1138 Huang JL, Li QB, Sun DH, Lu YH, Su YB, Yang X, Wang HX, Wang YP, Shao WY, He N, Hong JQ, Chen CX (2007) Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology 18:105104–105114 Ibrahim M, Alaam M, El-Haes H, Jalbout AF, Leon A (2006) Analysis of the structure and vibrational spectra of glucose and fructose. Ecl Quím 31:15–21 Talat M, Singh AK, Srivastava ON (2011) Optimization of process variables by central composite design for the immobilization of urease enzyme on functionalized gold nanoparticles for various applications. Bioprocess Biosyst Eng 34:647–657 Du L, Xian L, Feng J-X (2010) Rapid extra-/intracellular biosynthesis of gold nanoparticles by the fungus Penicillium sp. J Nanopart Res 13:921–930 Zhang JD, Chi QJ, Nielsen JU, Friis EP, Andersen JET, Ulstrup J (2000) Two-dimensional cysteine and cystine cluster networks on Au(111) disclosed by voltammetry and in situ scanning tunneling microscopy. Langmuir 16:7229–7237 Singh AK, Talat M, Singh DP, Srivastava ON (2010) Biosynthesis of gold and silver nanoparticles by natural precursor clove and their functionalization with amine group. J Nanopart Res 12:1667–1675 Kalwar NH, Nafady A, Sirajuddin Sherazi STH, Soomro RA, Hallam KR, Khaskheli AR, Jamali AA (2015) Catalytic degradation of imidacloprid using l-serine capped nickel nanoparticles. Mater Express 5:121–128 Mocanu A, Cernica I, Tomoaia G, Bobos L-D, Horovitz O, Tomoaia-Cotisel M (2009) Self-assembly characteristics of gold nanoparticles in the presence of cysteine. Colloid Surf A 338:93–101 Ayaz Ahmed KB, Subramanian S, Sivasubramanian A, Veerappan G, Veerappan A (2014) Preparation of gold nanoparticles using Salicornia brachiata plant extract and evaluation of catalytic and antibacterial activity. Spectrochim Acta A 130:54–58 Mishra A, Sardar M (2015) Cellulase assisted synthesis of nano-silver and gold: application as immobilization matrix for biocatalysis. Int J Biol Macromol 77:105–113 Rodrigues RC, Ortiz C, Berenguer-Murcia A, Torres R, Fernandez-Lafuente R (2013) Modifying enzyme activity and selectivity by immobilization. Chem Soc Rev 42:6290–6307 Abraham RE, Verma ML, Barrow CJ, Puri M (2014) Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass. Biotechnol Biofuels 7:90–101 Breger JC, Ancona MG, Walper SA, Oh E, Susumu K, Stewart MH, Deschamps JR, Medintz IL (2015) Understanding how nanoparticle attachment enhances phosphotriesterase kinetic efficiency. ACS Nano 9:8491–8503